Determination of Aflatoxins B1 in Tea by High Performance Liquid Chromatography-Fluorescence Detector with Post-column Photochemical Reaction

ZHAO Hao-jun; WANG Kun; YANG Wei-hua; YANG Chao-yi

doi:10.13305/j.cnki.jts.2013.03.001

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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (3) : 237-241. DOI: 10.13305/j.cnki.jts.2013.03.001

Determination of Aflatoxins B1 in Tea by High Performance Liquid Chromatography-Fluorescence Detector with Post-column Photochemical Reaction

ZHAO Hao-jun, WANG Kun^*, YANG Wei-hua, YANG Chao-yi

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Abstract

A new method for the sensitive determination of Aflatoxins B1 in tea by high performance liquid chromatography with photoelectric reactor and fluorescence detector was established. A solution of V(acetonitrile)∶V(H₂O)=86∶14 was used to extract Aflatoxins B1 from tea. The extracted solution was then purified by a multifunctional and immuneaffinity column, respectively. The peak area and the concentration of Aflatoxins B1 showed a good linear relationship within the range from 0.591βμg/L to 5.91βμg/L with a linear correlation coefficient (r) of 0.9994. The recoveries at the concentrations studied [low level (7.090βμg/kg), high level (14.180βμg/kg)] were between 85.6% and 98.9% with a relative standard deviations ranging from 1.7% to 1.8%. The limit of detection (LOD) is 0.1βμg/kg (S/N=3). The limit of quantitation (LOQ) is (0.591βμg/kg). The new method was used to analyze eight tea samples collected from the local markets and negative results were obtained. The method is suitable for detection of Aflatoxins B1 in tea with high selectivity and sensitivity.

Key words

Aflatoxins B1 / high performance liquid chromatography / immunoaffinity column / multifunctional purifying column / photochemical reactor / tea

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ZHAO Hao-jun, WANG Kun, YANG Wei-hua, YANG Chao-yi. Determination of Aflatoxins B1 in Tea by High Performance Liquid Chromatography-Fluorescence Detector with Post-column Photochemical Reaction[J]. Journal of Tea Science. 2013, 33(3): 237-241 https://doi.org/10.13305/j.cnki.jts.2013.03.001

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