EGCG抑制Nicotine诱导肺腺癌H1299细胞JAK2/STAT3 mRNA的表达研究

doi:10.13305/j.cnki.jts.2015.02.010

Abstract

Abstract: In order to explore the inhibitory effect of EGCG on nicotine which induced lung cancer cells proliferation, this study selected the optimal concentrations of EGCG and nicotine on lung adenocarcinoma cancer cell H1299 by MTT experiment, and detected mRNA relative expression levels of Bax, Bcl-2, Jak2 and Stat3 genes in H1299 cells using qRT-PCR technique. Experiment results showed that the IC₅₀ of EGCG on H1299 lung adenocarcinoma cells is about 32βμmol·L^-1 (treated 48βh) and 15βμmol·L^-1 (treated 24βh), nicotine obviously promotes the proliferation of H1299 lung adenocarcinoma cell at 1βμmol·L^-1 concentration treatment. Pretreatment of H1299 cells with 15βμmol·L^-1 EGCG (24βh) can significantly down-regulate the promotion of proliferation induced by 1βμmol·L^-1 Nicotine (P<0.05). Using 1βμmol·L^-1 nicotine to treat H1299 cells has showed that nicotine significantly reduce the mRNA expression level of Bax gene, increase the mRNA expression levels of Bcl-2, Jak2 and Stat3 genes in the JAK2/STAT3 signal pathway. Pretreatment of H1299 cells with 15βμmol·L^-1 EGCG (24βh) can be observed that EGCG significantly retroregulated the mRNA expression levels of Bax,Bcl-2,Jak2 and Stat3 genes in the JAK2/STAT3 signal pathway,and got the results with significant differences (P<0.05). In conclusion,EGCG plays important role in suppressing the survival rates of H1299 cells and mRNA expression level of JAK2/STAT3 signaling pathway induced by nicotine.

Key words: EGCG, nicotine, H1299 cell, JAK2/STAT3 mRNA expressions

CLC Number:

GAO Jin, YU Lijun, LI Xiaohuan, GAO Peng, ZHOU Qingming. Research on the Inhibitory Effect of EGCG on Nicotine in Inducing H1299 Cancer Cell JAK2/STAT3 mRNA Expression[J]. Journal of Tea Science, 2015, 35(2): 171-178.

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Research on the Inhibitory Effect of EGCG on Nicotine in Inducing H1299 Cancer Cell JAK2/STAT3 mRNA Expression

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