缺氮茶树暗处理下转录组分析及CsHY5基因的鉴定

doi:10.13305/j.cnki.jts.2026.02.014

Abstract

Abstract: Light and nitrogen are crucial factors regulating plant energy metabolism, carbon-nitrogen balance, and stress resistance. In this study, tea plant ‘Zhongming 7’ was subjected to dark treatment and normal light treatment under nitrogen-deficient conditions. The results indicate that dark treatment resulted in a decrease in SPAD values and a noticeable lightening of leaf color. Transcriptome analysis identifies 3 235 differentially expressed genes (DEGs), with 1 492 upregulated and 1 743 downregulated. KEGG enrichment analysis reveals significant enrichment of DEGs in pathways related to nitrogen metabolism, plant circadian rhythms and phytohormone signaling. Among the identified DEGs, an Elongated Hypocotyl 5 (CsHY5) gene was screened for further investigation. Protein-protein interaction network analysis reveals that CsHY5 primarily interacts with circadian rhythm-related genes. The expression level of the CsHY5 gene in new shoot tissues of three tea cultivars was analyzed using quantitative real-time PCR (RT-qPCR). The results reveals that the expression level of CsHY5 gene in the leaves of ‘Zhongming 7’ was higher than those observed in ‘Yunkang 10’ and ‘Hongya Foshou’. Transcriptome sequencing and RT-qPCR analysis indicate that the CsHY5 gene was downregulated under dark treatment. These findings suggest that CsHY5 may serve as a key node in the cross-regulation between light signaling and nitrogen metabolism, playing a crucial role in tea plants' response to combined dark treatment and nitrogen deficiency stress.

Key words: tea plant, CsHY5, darkness, nitrogen deficiency, transcriptome analysis

CLC Number:

S571.1
S326

WANG Yongxin, MAO Zhuozhuo, WEI Kang, WANG Liyuan, LU Wenyuan, YU Jizhong. Transcriptome Analysis of Nitrogen-Deficient Tea Plants Under Dark Treatment and Identification of the CsHY5 Gene[J]. Journal of Tea Science, 2026, 46(2): 255-263.

References

[1] Zhang Q F, Shi Y T, Hu H, et al.Magnesium promotes tea plant growth via enhanced glutamine synthetase-mediated nitrogen assimilation[J]. Plant Physiology, 2023, 192(2): 1321-1337.
[2] Wang Y X, Lei Y P, Wei K, et al.The effect of hypoxic stress on nitrate absorption and amino acid metabolism of tea plants[J]. Journal of Plant Growth Regulation, 2025, 44(11): 6467-6478.
[3] Zhang Y G, Ye X S, Zhang X W, et al.Natural variations and dynamic changes of nitrogen indices throughout growing seasons for twenty tea plant (Camellia sinensis L.) varieties[J]. Plants, 2020, 9(10): 1333. doi: 10.3390/plants9101333.
[4] Luo Q T, He H F.Accumulation of theanine in tea plant (Camellia sinensis (L.) O. Kuntze): biosynthesis, transportation and strategy for improvement[J]. Plant Science, 2025, 352: 112406. doi: 10.1016/j.plantsci.2025.112406.
[5] Chen Y Z, Wang F, Wu Z D, et al.Effects of long-term nitrogen fertilization on the formation of metabolites related to tea quality in subtropical China[J]. Metabolites, 2021, 11(3): 146. doi: 10.3390/metabo11030146.
[6] Wang Y, Wang Y M, Lu Y T, et al.Influence of different nitrogen sources on carbon and nitrogen metabolism and gene expression in tea plants (Camellia sinensis L.)[J]. Plant Physiology and Biochemistry, 2021, 167(6): 561-566.
[7] Shi H, Lü M, Luo Y W, et al.Genome-wide regulation of light-controlled seedling morphogenesis by three families of transcription factors[J]. PNAS, 2018, 115(25): 6482-6487.
[8] Ponnu J, Hoecker U.Illuminating the COP1/SPA ubiquitin ligase: fresh insights into its structure and functions during plant photomorphogenesis[J]. Fronts Plant Science, 2021, 12: 662793. doi: 10.3389/fpls.2021.662793.
[9] 李桂楠, 杨妮, 罗微, 等. CsDET2基因的鉴定及其对茶树光周期与非生物胁迫的响应分析[J]. 茶叶科学, 2025, 45(5): 742-756.
Li G N, Yang N, Luo W, et al.Identification of CsDET2 and its response analysis to photoperiod and abiotic stress in Camellia sinensis[J]. Journal of Tea Science, 2025, 45(5): 742-756.
[10] Wu Q J, Chen Z D, Sun W J, et al.De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan[J]. Fronts in Plant Science, 2016, 7: 662793. doi: 10.3389/fpls.2021.662793.
[11] Guan C L, Zhang D, Chu C C.Interplay of light and nitrogen for plant growth and development[J]. Crop Journal, 2025, 13(3): 641-655.
[12] Oliveira I C, Coruzzi G M.Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis[J]. Plant Physiology, 1999, 121(1): 301-309.
[13] Jonassen E M, Lea U S, Lillo C.HY5 and HYH are positive regulators of nitrate reductase in seedlings and rosette stage plants[J]. Planta, 2007, 227(3): 559-564.
[14] Gangappa S N, Botto J F.The multifaceted roles of HY5 in plant growth and development[J]. Molecular Plant, 2016, 9(10): 1353-1365.
[15] Stawska M, Oracz K.PhyB and HY5 are involved in the blue light-mediated alleviation of dormancy of seeds possibly via the modulation of expression of genes related to light, GA, and ABA[J]. International Journal of Molecular Sciences, 2019, 20(23): 5882. doi: 10.3390/ijms20235882.
[16] An J, Qu F, Yao J, et al.The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple[J]. Horticulture Research, 2017, 4: 17023. doi: 10.1038/hortres.2017.23.
[17] Ruan J Y, Gerendás J, Härdter R, et al.Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants[J]. Annals of Botany, 2007, 99(2): 301-310.
[18] Lillo C.Signalling cascades integrating light-enhanced nitrate metabolism[J]. Biochemical Journal, 2008, 415(1): 11-19.
[19] Jong F D, Thodey K, Lejay L V, et al.Glucose elevates NITRATE TRANSPORTER2.1 protein levels and nitrate transport activity independently of its HEXOKINASE1-MEDIATED stimulation of NITRATE TRANSPORTER2.1 expression[J]. Plant Physiology, 2013, 164(1): 308-320.
[20] 刘海光, 罗振, 董合忠. 植物硝态氮吸收和转运的调控研究进展[J]. 生物技术通报, 2021, 37(6): 192-201.
Liu H G, Luo Z, Dong H Z.Research progress on the regulation of NO₃- uptake and transport in plant[J]. Biotechnology Bulletin, 2021, 37(6): 192-201.
[21] Mu X H, Chen Y L.The physiological response of photosynthesis to nitrogen deficiency[J]. Plant Physiology and Biochemistry, 2021, 158: 76-82. doi: 10.1016/j.plaphy.2020.11.019.
[22] Chen X B, Yao Q F, Gao X H, et al.Shoot-to-root mobile transcription factor HY5 coordinates plant carbon and nitrogen acquisition[J]. Current Biology, 2016, 26(5): 640-646.
[23] Castillon A, Shen H, Huq E.Phytochrome interacting factors: central players in phytochrome-mediated light signaling networks[J]. Trends in Plant Science, 2007, 12(11): 514-521.
[24] Wang Q, Lin C T.Photoreceptor signaling: when COP1 meets VPs[J]. Embo Journal, 2019, 38(18): e102962. doi: 10.15252/embj.2019102962.
[25] Chakraborty M, Gangappa S N, Maurya J P, et al.Functional interrelation of MYC2 and HY5 plays an important role in Arabidopsis seedling development[J]. Plant Journal, 2019, 99(6): 1080-1097.
[26] Li J, Terzaghi W, Gong Y Y, et al.Modulation of BIN2 kinase activity by HY5 controls hypocotyl elongation in the light[J]. Nature Communications, 2020, 11(1): 1592. doi: 10.1038/s41467-020-15394-7.
[27] Yang G Q, Zhang C L, Dong H X, et al.Activation and negative feedback regulation of transcription by the SlBBX20/21-SlHY5 transcription factor module in UV-B signaling[J]. Plant Cell, 2022, 34(5): 2038-2055.
[28] Liu S Y, Wang Q L, Zhong M, et al.The CRY1-COP1-HY5 axis mediates blue-light regulation of Arabidopsis thermotolerance[J]. Plant Communications, 2025, 6(4): 101264. doi: 10.1016/j.xplc.2025.101264.

Transcriptome Analysis of Nitrogen-Deficient Tea Plants Under Dark Treatment and Identification of the CsHY5 Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN Zhiyin, ZENG Wenjuan, YANG Pan, WEN Cong, JIANG Rui, ZHONG Qianyi, JING Yuanrong, ZHU Zanjiang. Analysis of Mitochondrial Genome Characteristics and Codon Usage Bias in Camellia sinensis cv. ‘Zhuyeqi’ [J]. Journal of Tea Science, 2026, 46(2): 221-237.
[2]	LIU Enbei, WU Yedie, XU Miaomiao, LING Mingxing, PENG Jing, WANG Jie, WANG Xinchao, WANG Lu. Identification and Expression Regulation Analysis of the CsWCOR413 Gene Family in Camellia sinensis [J]. Journal of Tea Science, 2026, 46(1): 1-19.
[3]	ZHONG Lifan, HU Yunqing, ZHOU Qinyu, YANG Wang, WANG Li, LIU Zhen. Genetic Diversity Analysis and Core Germplasm Construction of Hunan Jietan Tea Based on Agronomic Traits and Whole Genome Sequencing [J]. Journal of Tea Science, 2026, 46(1): 35-49.
[4]	ZHANG Yunfan, ZHOU Fengjue, HU Junming, SONG Chuankui, ZHENG Fuhai, ZHANG Junhui, LI Tingting, LI Yuxiang. Plasma-Activated Sodium Lactate Enhances Secondary Metabolites and Physiological Resistance of Young Tea Plants [J]. Journal of Tea Science, 2026, 46(1): 61-72.
[5]	MA Siyu, LONG Lixue, LI Zilong, ZHAO Xianwang, HE Pengfei, HE Pengbo, CHEN Linbo, QU Hao, LONG Yaqin, TANG Ping. Isolation and Identification of the Pathogen Causing Anthracnose on Yunnan Large-Leaf Tea Plants and Screening of Antagonistic Bacteria [J]. Journal of Tea Science, 2026, 46(1): 89-100.
[6]	REN Ning, XUE Xianglei, ZHENG Hang, SHEN Shuai, YU Guohong. Design and Experimentation of a Pure Electric Cantilever Tea Plant Pruner [J]. Journal of Tea Science, 2026, 46(1): 111-121.
[7]	LI Yuanyuan, YAO Mingzhe, JIN Jiqiang. Elucidation of the Molecular Mechanisms Underlying the Formation of the High EGCG3′′Me Content in Tea Germplasms [J]. Journal of Tea Science, 2025, 45(6): 909-919.
[8]	SHEN Yingzi, LI Duojiao, HU Xingrong, JIANG Li, ZHENG Zhaisheng, LIU Huikang, YUAN Ming′an. Cloning of the CsPDAT1 Gene from Camellia sinensis and Its Role in Drought Tolerance [J]. Journal of Tea Science, 2025, 45(6): 920-930.
[9]	LU Li, SHI Yin, WANG Yanxia, HUANG Xiaozhen. Comparative Analysis of Seed Biological Characteristics and Endophytic Bacterial Diversity among Different Individual Plants of Ancient Tea Plants (Camellia sinensis) in Tongzi, Guizhou [J]. Journal of Tea Science, 2025, 45(6): 943-956.
[10]	CHEN Jiaming, GUO Yang, GU Dachuan, LI Jianlong, CHEN Yiyong, HUANG Yanfeng, TANG Jinchi, YANG Ziyin. Screening of Mechanical Harvestable Traits of Tea Cultivars [J]. Journal of Tea Science, 2025, 45(6): 1006-1020.
[11]	JIANG Li, LI Duojiao, HU Xinrong, SHEN Yingzi, ZHENG Zhaisheng, WENG Xiaoxing, LIU Shujing, BIAN Xiaodong, YUAN Ming'an, CHEN Xuan. Effects of Different Cultivation Patterns on Physiological and Biochemcial Characteristics of New Shoots in Seed-Leaf Dual-Purpose Tea Plants [J]. Journal of Tea Science, 2025, 45(5): 783-794.
[12]	WANG Kairong, ZHANG Longjie, LIANG Yuerong, LI Xiaoxiang, ZHENG Xinqiang. Identification and Classification of Tea Leaf Color and Establishment of A Tea Leaf Color System [J]. Journal of Tea Science, 2025, 45(5): 795-807.
[13]	ZHOU Yide, CHEN Jialin, WU Junmei, ZHAO Hongbo, SUN Binmei, LIU Shaoqun, ZHENG Peng. Nitrogen Metabolism Genes in Tea Plant: Research Progress on the Environmental Stress Adaptation Mechanism and Breeding Application [J]. Journal of Tea Science, 2025, 45(4): 545-558.
[14]	SUN Mengzhen, HU Zhihang, YANG Kaixin, ZHANG Jiaqi, ZHANG Nan, XIONG Aisheng, LIU Hui, ZHUANG Jing. Identification of Circadian Clock CsLUX Gene and Its Effects on Photosynthetic Characteristics in Tea Plants [J]. Journal of Tea Science, 2025, 45(4): 559-570.
[15]	ZHAI Xiuming, LI Jie, XIAO Fuliang, TANG Min, ZENG Lewu, HOU Yujia, TANG Yi. WGCNA Analysis of Differentially Expressed Genes between Parallel Variation and Normal Tea Stems and Leaves [J]. Journal of Tea Science, 2025, 45(3): 402-414.