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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (1): 65-70.doi: 10.13305/j.cnki.jts.2005.01.012

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Analysis of AcORF47 Homologue and VP80 Gene from a Newly Isolated Nucleopolyhedrovirus from the Tea Slug Moth, Iragoides fasciata

YANG Li-rong1, XIAO Qiang2, LENG Yang2, XU Hai-jun1, ZHANG Yi-qing1, ZHANG Chuan-Xi1, *   

  1. 1.Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Online:2005-03-25 Published:2019-09-16

Abstract: A baculovirus, Iragoides fasciata Nucleopolyhedrovirus(IrfaNPV)was newly isolated from the tea slug moth, Iragoides fasciata. The IrfaNPV AcORF47 homologue and VP80 gene were cloned. Sequence analysis showed IrfaNPV AcORF47 homologue shared 42%, 42% and 40% identities with AcMNPV ORF47, BmNPV ORF38 and RaouMNPV ORF44 in amino acid level, respectively. The 222 amino acid residues in the C-terminal of IrfaNPV VP80 had 25%~58% identities with homologous genes from other 17 NPVs. A phylogenetic tree was constructed based on the VP80/P87 amino acid sequences and the result showed that IrfaNPV was most closely related to AcMNPV, BmNPV and RaouMNPV.

Key words: Iragoides fasciata, Nucleopolyhedrovirus, AcORF47 homologue, Phylogeney, VP80

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