茶树MADS-box转录因子基因的克隆与非生物胁迫响应分析

Abstract

Abstract: In this study, based on the transcriptome database of tea plant, the CsMADS1 gene was cloned from cDNA of ‘Longjing43’ by RT-PCR method. The length of open reading frame of CsMADS1 gene was 657βbp, encoding 218 amino acids, which was a typical transcription factor of MADS-box family. Multiple alignments of CsMADS1 with related species showed that the identity of them was 65.65%, with a highly conserved MADS domain and a semi-conserved K domain. The physicochemical properties, hydrophilicity and hydrophobicity, subcellular localization, disordered feature, secondary and tertiary structure were also analyzed. CsMADS1 transcription factor is a hydrophilic protein, may be located in nucleus. The disordered feature of CsMADS1 protein was obvious, which was mainly composed of alpha helix structure, and had similar tertiary structure with MEF2 of human. Quantitative real-time PCR was used to analyze the expression profiles of the CsMADS1 gene under abiotic stress treatments of tea cultivar ‘Longjing43’. The results showed that the CsMADS1 gene responded to high and low temperatures, drought and high salinity. Different expression patterns of CsMADS1 gene were observed under those abiotic stress treatments.

Key words: Camellia sinensis, MADS-box, transcription factor, phylogenetic analysis, abiotic stress, expression analysis

CLC Number:

SHEN Wei, TENG Ruimin, LI Hui, LIU Zhiwei, WANG Yongxin, WANG Wenli, ZHUANG Jing. Cloning of a MADS-box Transcription Factor Gene from Camellia sinensis and its Response to Abiotic Stresses[J]. Journal of Tea Science, 2017, 37(6): 575-585.

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Cloning of a MADS-box Transcription Factor Gene from Camellia sinensis and its Response to Abiotic Stresses

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