普洱茶水提物与吡格列酮联合应用降糖功效研究

doi:10.13305/j.cnki.jts.2015.02.008

Abstract

Abstract: The aim of this study was to investigate the hypoglycemic effect of Pu′er tea extract combined with pioglitazone on diabetic model rat induced by STZ and clarify the synergistic effect of Pu′er tea extract with hypoglycemic drugs and the amount of hypoglycemic drugs could be reduced under the premise to control blood glucose. According to the blood glucose levels and the different ways of Pu′er tea extract combined with pioglitazone, all animals were divided into different groups including control, model, high P, low P, HP+LPTE, HP+HPTE, LP+LPTE, LP+HPTE, orally administered twice a day for consecutive 4 weeks. Animals’ status was observed during the test, and rat tail veins were weekly acupunctured to measure fasting blood glucose. The glucose tolerance, GSP and insulin levels in serum were determined in fourth week and insulin sensitivity index was calculated. The results showed that Pu′er tea extract combined with mixed medication pioglitazone could significantly suppress fasting blood glucose, lower blood glucose at 30βmin, 60βmin, 120βmin after administration of glucose, and reduce the AUC as compared with other groups. Additionally, serum GSP levels were also reduced after treatment with Pu′er tea extract combined with pioglitazone. These results suggest that pioglitazone combined with pu′er tea extract could reduce the amount of pioglitazone without reducing pioglitazone’s hypoglycemic effect.

Key words: Pu′er tea extract;, hypoglycemic, drug combination, pioglitazone, tolerance

CLC Number:

TS272.5⁺4

HU Jinfang, WANG Genbei, XU Yan, MA Xiaohui, LI Changwen, SHEN Xiuping. The Study of Hypoglycemic Effect of Pu′er Tea Extract Combined with Pioglitazone[J]. Journal of Tea Science, 2015, 35(2): 158-164.

References

[1] HsiKao Y, Chang H H, Lee M J, et al. Review tea, obesity, and diabetes [J]. Molecular Nutrion Food Research. 2006, 50(2): 188-210.
[2] Lee L K, Foo K Y.Recent advances on the beneficial use and health implications of Pu-Erh tea[J]. Food Research International, 2013, 53(2): 619-628.
[3] 陈浩. 普洱茶多糖降血糖及抗氧化作用研究[D]. 杭州: 浙江大学, 2013.
[4] 张冬英, 邵宛芳, 刘仲华, 等. 普洱茶化学成分及对α-淀粉酶抑制作用的研究[J]. 西南农业学报, 2009, 22(1): 52-54.
[5] 吕海鹏. 普洱茶化学成分与抗氧化活性研究[D]. 北京: 中国农业科学院, 2005.
[6] 罗一鸣, 周芝芹. 13种茶叶中多糖的含量分析[J]. 湖南医科大学学报, 1996, 21(4): 298-300.
[7] 李布青, 张慧玲, 舒庆龄, 等. 中低档绿茶中茶多糖的提取及降血糖作用[J]. 茶叶科学, 1996, 16(1): 67-72.
[8] 陈海霞, 谢笔军. 茶多糖不同提取工艺的比较研究[J]. 食品工业科技, 2003, 22(2): 18.
[9] 周志, 汪兴平, 张家年. 茶多糖分离提取技术研究[J]. 食品与发酵工业, 2001, 28(3): 83-84.
[10] 倪德江, 谢笔钧, 宋春和. 不同茶类多糖对实验型糖尿病小鼠治疗作用的比较研究[J]. 茶叶科学, 2002, 22(2): 376-381.
[11] 周斌星, 孔令波, 陈军贤. 普洱茶多糖的提取及其降血糖研究[J]. 中国农学通报, 2009, 25(15): 55-59.
[12] 庞烨峰. 吡格列酮对1型糖尿病大鼠胰岛素抵抗的改善及机制研究[D]. 长沙: 湖南大学, 2013.
[13] 王自春, 王征. 金芪降糖片联合吡格列酮片治疗2型糖尿病高胰岛素血症的疗效观察[J]. 天津医药, 2013, 41(11): 1122-1123.
[14] 栗志文, 王媛媛, 王根辈, 等. 普洱茶提取物与绿茶提取物降糖功效的研究[J]. 茶叶科学, 2014, 34(5): 428-434.
[15] 丁仁凤,何普明,揭国良. 茶多糖和茶多酚的降血糖作用研究[J]. 茶叶科学,2005, 25(3): 219-224.
[16] 全吉淑,尹学哲,及川志和. 茶多糖降糖作用机制[J]. 中国公共卫生,2007, 23(3): 295-296.
[17] Kim MK, Jung HS, Yoon CS, et al. EGCG and quercetin protected INS-1 cells in oxidative stress via different mechanisms[J]. Front Biosci, 2010(2): 810-817.
[18] 王立波, 梁名志, 付学奇, 等. 普洱茶发酵前后对PTPs靶标抑制效果的比较[J]. 氨基酸和生物资源, 2010, 32(2): 20-22.

The Study of Hypoglycemic Effect of Pu′er Tea Extract Combined with Pioglitazone

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