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Study on Composite-enzymolysis Assisted Ethanol Extraction of Tea Polyphenols

  • XIE Lan-hua ,
  • XIAO Nan ,
  • ZHANG Jia-yi ,
  • JIANG Dong-wen ,
  • DU Bing ,
  • YANG Gong-ming
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  • College of Food, South China Agricultural University, Guangzhou 510642, China

Received date: 2012-04-05

  Revised date: 2012-05-22

  Online published: 2019-09-04

Abstract

Composite-enzymolysis assisted ethanol extraction of tea polyphenols from Danzong-tea was optimized in this investigation. The effects of factors including enzymolysis temperature, enzyme content, enzymolysis pH, enzymolysis time, ethanol concentration, solid-to-liquid ratio,extraction temperature, extraction time on the extraction rates of tea polyphenols were explored by single-factor and orthogonal test. The results showed that the optimal extraction conditions were after enzyming pretreatment in the conditions of enzymatic content 3.5%, enzymolysis temperature 60℃, enzymolysis pH 4.8, enzymolysis time 3 h, then 60% ethanol to extract for 1.5 h with extraction temperature70℃ and solid-to-liquid ratio 1︰50, tea polyphenols extraction in Danzong-tea was up to 25.82% and ncreased 69.87% compared with the traditional water extraction.

Cite this article

XIE Lan-hua , XIAO Nan , ZHANG Jia-yi , JIANG Dong-wen , DU Bing , YANG Gong-ming . Study on Composite-enzymolysis Assisted Ethanol Extraction of Tea Polyphenols[J]. Journal of Tea Science, 2013 , 33(1) : 27 -33 . DOI: 10.13305/j.cnki.jts.2013.01.001

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