Study on Composite-enzymolysis Assisted Ethanol Extraction of Tea Polyphenols

XIE Lan-hua; XIAO Nan; ZHANG Jia-yi; JIANG Dong-wen; DU Bing; YANG Gong-ming

doi:10.13305/j.cnki.jts.2013.01.001

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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (1) : 27-33. DOI: 10.13305/j.cnki.jts.2013.01.001

Study on Composite-enzymolysis Assisted Ethanol Extraction of Tea Polyphenols

XIE Lan-hua, XIAO Nan, ZHANG Jia-yi, JIANG Dong-wen, DU Bing^*, YANG Gong-ming

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Abstract

Composite-enzymolysis assisted ethanol extraction of tea polyphenols from Danzong-tea was optimized in this investigation. The effects of factors including enzymolysis temperature, enzyme content, enzymolysis pH, enzymolysis time, ethanol concentration, solid-to-liquid ratio,extraction temperature, extraction time on the extraction rates of tea polyphenols were explored by single-factor and orthogonal test. The results showed that the optimal extraction conditions were after enzyming pretreatment in the conditions of enzymatic content 3.5%, enzymolysis temperature 60℃, enzymolysis pH 4.8, enzymolysis time 3 h, then 60% ethanol to extract for 1.5 h with extraction temperature70℃ and solid-to-liquid ratio 1︰50, tea polyphenols extraction in Danzong-tea was up to 25.82% and ncreased 69.87% compared with the traditional water extraction.

Key words

composite- enzymolysis / ethanol extraction / tea polyphenols

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XIE Lan-hua, XIAO Nan, ZHANG Jia-yi, JIANG Dong-wen, DU Bing, YANG Gong-ming. Study on Composite-enzymolysis Assisted Ethanol Extraction of Tea Polyphenols[J]. Journal of Tea Science. 2013, 33(1): 27-33 https://doi.org/10.13305/j.cnki.jts.2013.01.001

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