By the experimental procedure of enrichment culture and microbial purification, 33 ammonifying bacterial strains were screened from the soil of tea garden in Shandong province. According to the activity comparison and taxonomy of strains, three different species of high activity strains were selected. The strain SNT3 was preliminary identified as Bacillus, SNT6 as Exiguobacterium and SNT33 as Acinetobacter on the basis of the characteristics of strain’s morphological characteristics, physiological＆biochemistry and 16βS rDNA sequencing method. Meanwhile, the effects of growth environment and removal ability of organic nitrogen of the strains were measured . The results showed that the optimum pH for SNT3, SNT6, SNT33 is 5.0~8.5, 6.0~8.5, 5.0~7 respectively; the optimum temperature of three strains were 35℃, the capacity of organic nitrogen removing of the SNT3 was better than SNT6 and SNT33.
HAN Xiao-yang
,
LI Zhi
,
WANG Qiang-qiang
,
ZHANG Li-xia
. Research on Screening and Identification of Ammonifying Bacterium and Characteristic of Strains from the Soils of Tea Garden[J]. Journal of Tea Science, 2013
, 33(1)
: 91
-98
.
DOI: 10.13305/j.cnki.jts.2013.01.012
[1] 高美英, 刘和, 冀常军, 等. 覆盖对果园土壤氨化细菌数量年变化的影响[J]. 土壤通报, 2000, 31(6): 273-274.
[2] Yu Liu-qing, Zhu Yong-lin, Wu Lin-fu, et al. Effeet of Herbicides and Their Mixture with Urea on Ammonifying Bacteria in Paddy field[J]. Chinese J Rice Sci, 1997, 11(l): 44-46.
[3] 赵斌, 何绍江. 微生物学实验[M]. 北京: 科学出版社, 2008.
[4] 陈佳荣. 水化学实验指导书[M]. 北京: 中国农业出版社, 1996.
[5] Laemmli U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature, 1970, 227, 680-685.
[6] 谭志远, 陈文新. 根瘤菌新类群的全细胞蛋白电泳及16S rDNA全序列分析[J]. 应用与环境生物学报, 1998, 4(1): 65-69.
[7] Tan Z Y, Kan F L, Peng G X, et al. Rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in China[J]. Int J Syst Evol Microbiol, 2001, 51: 909-914.
[8] Weisburgw G, Bams M, Elletier D A.16sRibosomal DNA Amplification for Phylogenetic Study[J]. J Bacterial, 1991, 173(2): 697-703.
[9] Buchanan R E, Gibbons N E.伯杰细菌鉴定手册[M]. 8版. 北京: 科学出版社, 1984.
[10] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京科学出版社, 2001.
[11] 王华荣, 彭桂香, 张国霞, 等. 糖蜜草(Melinis minutiflora Beauv.)内生固氮菌分离鉴定[J]. 生态学报, 2006, 26(8): 2566-2571.
[12] 闫爱民, 陈文新. 干旱地区几种豆科植物根瘤菌的数值分类和SDS-PAGE全细胞蛋白电泳分析[J]. 应用与环境生物学报, 1998, 4(4): 354-359.
[13] 王娟, 戴习林, 宋增福. 一株氨化细菌的分离、鉴定及氨氮降解能力的初步分析[J]. 水生生物学报, 2010, 34(6): 1198-1201.
[14] Collins M.D, Lund B M, Farrow J A E, et al. Chemotaxonomic study of an alkalophilic bacterium, Exiguobacterium aurantiacum gen. nov, sp. nov[J]. J Gen Microbiol, 1983, 129: 2037-2042.
[15] Knudston KE, Haas EJ, Iwen PC, et al. Characterization of a Gram-positive non spore forming Exiguobacterium like organism isolated from a western Colorado (USA) hot spring[J]. Abstr: Annu Meet Am Soc Microbiol, 2001, 1(92): 30.
[16] Reiter B, Pfeifer U, Schwab H, et al. Response of endophytic bacterial communities in potato plant to infection with Erwinia carotovora subsp. Atroseptica[J]. Appl Environ Microbiol, 2002, 68: 2261-2268.
[17] Miteva VI, Sheridan PP, Brenchly JE.Phylogenetic and physiological diversity of microorganisms isolated from a deep Greenland glacier ice core[J]. Appl Environ Microbiol, 2004, 70: 202-213.
[18] Rodriguez DF, Goris J, Vishnivetskaya et al. Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov[J]. Extremophiles, 2006, 10: 285-294.
[19] Govindan Selvakumar, Piyush Joshi, Sehar Nazim, et al. Exiguobacterium acetylicum strain 1P (MTCC 8707) a novel bacterial antagonist from the North Western Indian Himalayas[J]. World J Microbiol Biotechnol, 2009, 25: 131-137.
[20] 史亚萍, 张宏, 郭道森, 等. 一种促进蓝藻生长的海洋细菌的分离与鉴定[J]. 青岛大学学报: 工程技术版, 2010, 25(2): 41-46.
[21] 胡江, 代先祝, 李顺鹏. 阿特拉津降解菌BTAH1的分离与鉴定[J]. 中国环境科学, 2004, 24(6): 738-742.
[22] 王琳, 李季, 郭廷忠, 等. 乙酸钙不动杆菌对富营养化景观水体的净化作用[J]. 生态环境, 2008, 17(5): 1784-1786.
[23] 赵菁, 黄奋飞, 林生, 等. 纤维素降解细菌的分离、鉴定及其系统发育分析[J]. 安徽农学通报, 2011, 17(17): 47-48, 81.
[24] 段星春, 易筱筠, 党志, 等. 乙酸钙不动杆菌TS2H对DBP降解特性的研究[J]. 生态环境, 2007, 16(3): 846-849.
[25] 施国涵. 松树和杨树林下土壤微生物对灭幼脲3号降解代谢的研究[J]. 林业科学, 1991, 27(4): 452-458.
浙公网安备 33019902000101号 
