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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (1): 91-98.doi: 10.13305/j.cnki.jts.2013.01.012

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Research on Screening and Identification of Ammonifying Bacterium and Characteristic of Strains from the Soils of Tea Garden

HAN Xiao-yang1,2, LI Zhi1,2, WANG Qiang-qiang1,2, ZHANG Li-xia1,2,*   

  1. 1. College of Horticulture Science and Engineering, Shandong Agriculture University;
    2. State key laboratory of Crop Biology, Taian 271018, China
  • Received:2012-06-11 Revised:2012-08-14 Online:2013-02-28 Published:2019-09-04

Abstract: By the experimental procedure of enrichment culture and microbial purification, 33 ammonifying bacterial strains were screened from the soil of tea garden in Shandong province. According to the activity comparison and taxonomy of strains, three different species of high activity strains were selected. The strain SNT3 was preliminary identified as Bacillus, SNT6 as Exiguobacterium and SNT33 as Acinetobacter on the basis of the characteristics of strain’s morphological characteristics, physiological&biochemistry and 16βS rDNA sequencing method. Meanwhile, the effects of growth environment and removal ability of organic nitrogen of the strains were measured . The results showed that the optimum pH for SNT3, SNT6, SNT33 is 5.0~8.5, 6.0~8.5, 5.0~7 respectively; the optimum temperature of three strains were 35℃, the capacity of organic nitrogen removing of the SNT3 was better than SNT6 and SNT33.

Key words: tea garden soil, ammonifying bacterium, identification, characteristic research

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