Ligand exchange chromatography with chiral mobile phases was established for the separation of theanine enantiomers.The optimum condition was :Polaris C18 column, mobile phase: L-proline: Cu2+=2:1, the concentration of Cu2+ was 0.5βmmol/L, pH6.8, the volume of methanol is 2%, wavelength: 254βnm;flow rate:0.9βml/min, column temperature:30℃. There was a good linearity between peak areas and injection quantities of L-Theanine ranging from 0.09542βμg~4.241βμg and D-Theanine ranging from 0.08486βμg~4.243βμg. The recovery of L-Theanine range from 97.45%~100.4% and D-Theanine range from 97.07%~100.1%.
LI Yin-hua
,
LIU Zhong-hua
,
HUANG Jian-an
. Separation and Quantitation of Theanine Enantiomers on Ligand Exchange Chromatography with Chiral Mobile Phase[J]. Journal of Tea Science, 2006
, 26(4)
: 280
-284
.
DOI: 10.13305/j.cnki.jts.2006.04.009
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