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Journal of Tea Science ›› 2006, Vol. 26 ›› Issue (4): 280-284.doi: 10.13305/j.cnki.jts.2006.04.009

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Separation and Quantitation of Theanine Enantiomers on Ligand Exchange Chromatography with Chiral Mobile Phase

LI Yin-hua, LIU Zhong-hua*, HUANG Jian-an   

  1. Institute of Natural Products, Hunan Agricultural University, Changsha 410128, China
  • Received:2006-07-05 Revised:2006-08-28 Online:2006-12-25 Published:2019-09-11

Abstract: Ligand exchange chromatography with chiral mobile phases was established for the separation of theanine enantiomers.The optimum condition was :Polaris C18 column, mobile phase: L-proline: Cu2+=2:1, the concentration of Cu2+ was 0.5βmmol/L, pH6.8, the volume of methanol is 2%, wavelength: 254βnm;flow rate:0.9βml/min, column temperature:30℃. There was a good linearity between peak areas and injection quantities of L-Theanine ranging from 0.09542βμg~4.241βμg and D-Theanine ranging from 0.08486βμg~4.243βμg. The recovery of L-Theanine range from 97.45%~100.4% and D-Theanine range from 97.07%~100.1%.

Key words: HPLC, theanin enantiomer, separation, chiral mobile phases

CLC Number: