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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (3): 218-224.doi: 10.13305/j.cnki.jts.2011.03.003

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Study on Extraction and SeparationProcess of EGCG3˝Me

LUO Zheng-fei1,2, JIN Xiao-fang2,3, GONG Zheng-li2*, LIANG Min-zhi1, WANG Yi4, WANG Li-bo1,2, CHEN Ji-wei1   

  1. 1. College of Food Science, Southwest University, Chongqing 400716, China;
    2. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China;
    3. Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430209, China;
    4. Chongqing Great Wall Tea Trade Company, Chongqing 400020, China
  • Received:2010-08-25 Revised:2010-12-30 Online:2011-06-15 Published:2019-09-06

Abstract: :The extraction and separation process of EGCG3˝Me was studied by HPLC, polyamide resin and HPTLC methods. According to the orthogonal experimental design, the optimum extracting conditions of EGCG3˝Me were as follows: alcohol concentration 70%, reflux time 30 min, reflux temperature 80℃ and solid-liquid ratio 1:10. And the yield of EGCG3˝Me was 0.89%. Large amount of EGCG3˝Me was found in the elution of alcohol concentration 40% and 60%. With elution of methanol: chloroform (7:2), the high purity EGCG3˝Me was obtained by further separation and purification of 100~120 mesh polyamide resin.

Key words: EGCG3˝Me, extraction, separation, HPLC, polyamide

CLC Number: