EGCG3˝Me提取与分离工艺研究

doi:10.13305/j.cnki.jts.2011.03.003

Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (3): 218-224.doi: 10.13305/j.cnki.jts.2011.03.003

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Study on Extraction and SeparationProcess of EGCG3˝Me

LUO Zheng-fei^1,2, JIN Xiao-fang^2,3, GONG Zheng-li^2*, LIANG Min-zhi¹, WANG Yi⁴, WANG Li-bo^1,2, CHEN Ji-wei¹

1. College of Food Science, Southwest University, Chongqing 400716, China;
2. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China;
3. Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430209, China;
4. Chongqing Great Wall Tea Trade Company, Chongqing 400020, China

Received:2010-08-25 Revised:2010-12-30 Online:2011-06-15 Published:2019-09-06

Abstract

Abstract: ：The extraction and separation process of EGCG3˝Me was studied by HPLC, polyamide resin and HPTLC methods. According to the orthogonal experimental design, the optimum extracting conditions of EGCG3˝Me were as follows: alcohol concentration 70%, reflux time 30 min, reflux temperature 80℃ and solid-liquid ratio 1:10. And the yield of EGCG3˝Me was 0.89%. Large amount of EGCG3˝Me was found in the elution of alcohol concentration 40% and 60%. With elution of methanol: chloroform (7:2), the high purity EGCG3˝Me was obtained by further separation and purification of 100~120 mesh polyamide resin.

Key words: EGCG3˝Me, extraction, separation, HPLC, polyamide

CLC Number:

LUO Zheng-fei, JIN Xiao-fang, GONG Zheng-li, LIANG Min-zhi, WANG Yi, WANG Li-bo, CHEN Ji-wei. Study on Extraction and SeparationProcess of EGCG3˝Me[J]. Journal of Tea Science, 2011, 31(3): 218-224.

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Study on Extraction and SeparationProcess of EGCG3˝Me

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