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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (3): 397-406.doi: 10.13305/j.cnki.jts.2020.03.010

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Determination of Pesticide Residue in Fresh Tea Leaves and Dry Tea by Solid Extraction and Dispersive Solid Extraction Cleanup Coupled with Tandem Mass Spectrum

YANG Jie1,2, ZHOU Li1,*, YU Huan1,2, SUN Hezhi1, WANG Xinru1, ZHANG Xinzhong1, YANG Mei1, CHEN Zongmao1, LUO Fengjian1,*   

  1. 1. Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-09-27 Revised:2020-01-04 Online:2020-06-15 Published:2020-06-09

Abstract: A method for the determination of 9 pesticide residues in fresh and dry tea was established. Residual pesticides in samples were extracted by acetonitrile, purified by Florisil/GCB solid phase extraction column, followed by absorbent of PSA and GCB as dispersed solid phase, and determined by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS). In the spiked range of 0.005-1.000 mg·kg-1, the average recoveries of target pesticides in fresh leaves and dried tea were 70.3%-103.9%, and the relative standard deviations (RSD) were less than 20%. In the range of 0.005-2.000 mg·kg-1, the linear relationship of target pesticides in fresh leaves and dry tea matrix was good, with r>0.995 4. The limit of quantity (LOQ) was 0.005 mg·kg-1. The test results of actual samples showed that the method was highly sensitive and reproducible, and can meet the requirements of multi-residue detection.

Key words: multi-residue of pesticides, UPLC-MS/MS, GC-MS, tea, solid phase extraction, dispersive solid-phase extraction

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