Abstract: The separation of procyanidins(PC) from episperm of tea (Camellia sinensis) seeds was separated by using macroporous absorption resin of column of HPD200A materials, and the obtained PC was qualitatively determined by color reaction of anthocyanin, UV-scanning, and thin layer chromatographic method, and quantitatively determined by HPLC method. Results showed that both the sample I and II eluted by 20% ethanol and 60% ethanol were the PC, and their obtained rate were 15.00mg/g and 36.90mg/g, purity 97.22% and 76.46%, respectively. Additionally, sample I contained 0.05% D,L-C, 0.35% EGCG and 0.02% theobromine by HPLC analysis while sample II contained 0.01% EGCG, 0.02% theobromine and 0.07 % caffeine, respectively.

Key words: tea seed episperm, proanthocyanidins, separation of column, qualitative analysis, quantitative analysis