茶籽壳原花青素的鉴定与分析

doi:10.13305/j.cnki.jts.2011.02.007

Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (2): 119-123.doi: 10.13305/j.cnki.jts.2011.02.007

Previous Articles Next Articles

Identification and Analysis of Procyanidins from Episperm of Tea (Camellia sinensis) Seeds

DONG Rui-xia^1,2, LI Li-xiang^1*, JIANG Qi-zhong¹

1. Key Lab of Tea Biochemistry & Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agriculture University, Hefei 230036, China;
2. School of Environment Engineering, Lishui Vocational & Technical College, Lishui 323000, China;

Received:2010-05-20 Revised:2010-09-25 Online:2011-04-15 Published:2019-09-06

Abstract

Abstract: The separation of procyanidins(PC) from episperm of tea (Camellia sinensis) seeds was separated by using macroporous absorption resin of column of HPD200A materials, and the obtained PC was qualitatively determined by color reaction of anthocyanin, UV-scanning, and thin layer chromatographic method, and quantitatively determined by HPLC method. Results showed that both the sample I and II eluted by 20% ethanol and 60% ethanol were the PC, and their obtained rate were 15.00mg/g and 36.90mg/g, purity 97.22% and 76.46%, respectively. Additionally, sample I contained 0.05% D,L-C, 0.35% EGCG and 0.02% theobromine by HPLC analysis while sample II contained 0.01% EGCG, 0.02% theobromine and 0.07 % caffeine, respectively.

Key words: tea seed episperm, proanthocyanidins, separation of column, qualitative analysis, quantitative analysis

CLC Number:

DONG Rui-xia, LI Li-xiang, JIANG Qi-zhong. Identification and Analysis of Procyanidins from Episperm of Tea (Camellia sinensis) Seeds[J]. Journal of Tea Science, 2011, 31(2): 119-123.

[1]	LIU Yajun, WANG Peiqiang, JIANG Xiaolan, ZHUANG Juhua, GAO Liping, XIA Tao. Research Progress on the Biosynthesis of Monomeric and Polymeric Catechins in Camellia sinensis [J]. Journal of Tea Science, 2022, 42(1): 1-17.
[2]	GAO Chenxi, HUANG Yan, SUN Weijiang. Research Progress of Proanthocyanidins in Tea [J]. Journal of Tea Science, 2020, 40(4): 441-453.
[3]	CHEN Mei-li, ZHANG Jun, GONG Shu-ying, TANG De-song, ZHANG Ying-bin, GU Zhi-lei. Establishment of Predictive Model for Quantitative Analysis of Major Components in Jasmine Tea by Near Infrared Spectroscopy(NIRS) [J]. Journal of Tea Science, 2013, 33(1): 21-26.
[4]	WANG Yu-xia, XU Rong-rong, REN Guang-xin, WANG Sheng-peng, WAN Xiao-chun, ZHANG Zheng-zhu. Establishment of the Predictive Models for Quantitative Analysis of Major Components in Green Tea Infusion by Near Infrared Spectroscopy (NIRS) [J]. Journal of Tea Science, 2011, 31(4): 355-361.
[5]	ZHOU Jian, CHENG HaoP, WANG Li-yuan. Recent Advance on the Application of Near-infrared Spectroscopy in Tea [J]. Journal of Tea Science, 2008, 28(4): 294-300.

Identification and Analysis of Procyanidins from Episperm of Tea (Camellia sinensis) Seeds

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0