伐地那非增加EGCG-β-乳球蛋白纳米粒对肝癌细胞的抑制作用

doi:10.13305/j.cnki.jts.2020.04.009

茶叶科学 ›› 2020, Vol. 40 ›› Issue (4): 528-535.doi: 10.13305/j.cnki.jts.2020.04.009

伐地那非增加EGCG-β-乳球蛋白纳米粒对肝癌细胞的抑制作用

陈春晓, 楼文雨, 丁镇建, 李卓烨, 杨媛媛, 金鹏, 杜琪珍^*

浙江农林大学农业与食品科学学院,浙江杭州 311300

收稿日期:2019-10-09 修回日期:2020-04-11 出版日期:2020-08-15 发布日期:2020-08-18
通讯作者: *qizhendu@163.com
作者简介:陈春晓，女，硕士研究生，主要从事天然活性物质方面的研究。
基金资助:
浙江省重点研发（2019C02072）、浙江农林大学大学生创新（113-2013200135、115-2013200021）

Vardenafil Improves the Proliferative Inhibition of EGCG-β-lactoglobulin Nanoparticles Against Liver Cancer Cells

CHEN Chunxiao, LOU Wenyu, DING Zhenjian, LI Zhuoye, YANG Yuanyuan, JIN Peng, DU Qizhen^*

School of Agricultural and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China

Received:2019-10-09 Revised:2020-04-11 Online:2020-08-15 Published:2020-08-18

摘要/Abstract

摘要： 较高浓度的EGCG才能抑制癌细胞的增殖,通过纳米化和EGCG与其他药物的联合使用是提高EGCG生物活性的重要策略。本研究将EGCG和伐地那非（VD）同时包埋于β-乳球蛋白（β-Lg）纳米载体中,制备出EGCG-VD-β-Lg纳米粒（EVβ-NPs）,体外试验证实,EVβ-NPs能提高人肝癌细胞（HepG2细胞）中Caspase-3活性,使HepG2细胞在S期产生明显的阻滞,诱发细胞核分裂,从而导致HepG2细胞凋亡。研究结果表明,将EGCG与微量的VD联合使用,并通过纳米化包埋可以显著提高EGCG的抗癌活性。这一方法在EGCG抗癌制品的开发方面具有潜在的价值。

关键词: 表没食子儿茶素没食子酸酯, 伐地那非, β-乳球蛋白;, 纳米粒, 增殖抑制

Abstract: The combination of nano and other drugs is an important strategy to improve the biological activity of EGCG, since a high EGCG concentration is essential for the inhibition of the proliferation of cancer cells. In this study, EGCG-vardenafil (VD)-β-lactoglobulin (β-Lg)-nanoparticles (EVβ-NPs) was prepared by encapsulating EGCG and VD in β-Lg nano-carriers. In vitro experimental results show that EVβ-NPs could upgrade the activity of caspase-3 in HepG2 cells compared to the native EGCG, which caused cell cycle arrest in the S phase of HepG2 cells to induce cell nuclear division, and finally lead to HepG2 cell apoptosis. The results demonstrate that the encapsulation of EGCG-VD can significantly improve the anticancer activity of EGCG, and possesses potential value in the development of EGCG anticancer products.

Key words: EGCG, vardenafil, β-lactoglobulin, nanoparticles, proliferative inhibition

中图分类号:

陈春晓, 楼文雨, 丁镇建, 李卓烨, 杨媛媛, 金鹏, 杜琪珍. 伐地那非增加EGCG-β-乳球蛋白纳米粒对肝癌细胞的抑制作用[J]. 茶叶科学, 2020, 40(4): 528-535. doi: 10.13305/j.cnki.jts.2020.04.009.

CHEN Chunxiao, LOU Wenyu, DING Zhenjian, LI Zhuoye, YANG Yuanyuan, JIN Peng, DU Qizhen. Vardenafil Improves the Proliferative Inhibition of EGCG-β-lactoglobulin Nanoparticles Against Liver Cancer Cells[J]. Journal of Tea Science, 2020, 40(4): 528-535. doi: 10.13305/j.cnki.jts.2020.04.009.

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伐地那非增加EGCG-β-乳球蛋白纳米粒对肝癌细胞的抑制作用

Vardenafil Improves the Proliferative Inhibition of EGCG-β-lactoglobulin Nanoparticles Against Liver Cancer Cells

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