Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers

YAO Ming-zhe; LIU Zhen; CHEN Liang; WANG Xin-chao; MA Chun-lei; LIANG Yue-rong

doi:10.13305/j.cnki.jts.2009.3.011

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (3) : 243-250. DOI: 10.13305/j.cnki.jts.2009.3.011

Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers

YAO Ming-zhe^1,2, LIU Zhen², CHEN Liang^2,*, WANG Xin-chao², MA Chun-lei², LIANG Yue-rong^1,*

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Abstract

The 25 pairs of EST-SSR primers were used to analyze the genetic diversity, genetic structure and genetic relationship of 45 tea accessions originated from region of north Yangtze River. Totally 83 alleles were identified, on an average of 3.3 alleles each pair of primers. The mean of polymorphism information content (PIC) was 0.61. The observed heterozygosity (Ho) was averagely higher than the expected heterozygosity (He). Among 45 tea accessions the average numbers of the observed alleles were 4.2, and the number of effective alleles 2.8. The observed heterozygosity, gene diversity and Shannon information index were 0.73, 0.61 and 1.11, respectively. The low genetic difference （Gst=0.2） and high geneflow (Nm=3.9) of tea populations from Hubei, Anhui and Shaanxi were found. Analysis of molecular variance (AMOVA) showed that variance components inter-population and intra-population were 4.03% and 95.97% respectively. The paired similarity coefficients of 45 accessions were from 0.32 to 0.89. No clearly regional partition among 45 accessions was found from the dendrogram. The high level of genetic similarity was demonstrated among tea population of Hubei, Anhui and Shaanxi province, and Shaanxi population showed relatively distant relationship comparing to population of Hubei and Anhui.

Key words

Camellia sinensis / EST-SSR / genetic diversity / genetic structure / tea

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YAO Ming-zhe, LIU Zhen, CHEN Liang, WANG Xin-chao, MA Chun-lei, LIANG Yue-rong. Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers[J]. Journal of Tea Science. 2009, 29(3): 243-250 https://doi.org/10.13305/j.cnki.jts.2009.3.011

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