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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (1): 24-34.doi: 10.13305/j.cnki.jts.2015.01.006

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The Cloning of Transcription Factor Gene CsDREB-A4 and The Response to Temperature Stress in Camellia sinensis

LIU Zhiwei1,3, XIONG Yangyang2, LI Tong3, YAN Yajun3, HAN Hongrun3, WU Zhijun1,3, ZHUANG Jing1,3,*   

  1. 1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
    2. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;
    3. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2014-07-15 Revised:2014-08-21 Published:2019-08-23

Abstract: The CsDREB-A4 gene, which encoding to the DREB transcription factor, was cloned by PCR using DNA as template from tea plant (Camellia sinensis) cultivar ‘Yingshuang’, based on transcriptome data of ‘Yingshuang’. The open reading frame lengths of CsDREB-A4 gene was 708βbp, encoding 235 amino acids. The transcription factor of CsDREB-A4 contained the AP2 DNA binding domain, and had high homology with DERB transcription factors of Glycine max, Solanum lycopersicum, Vitis vinifera, Arabidopsis thaliana, and so on. Phylogenetic tree, hydrophilicity, disordered residues, two- and three-dimension structure of CsDREB-A4 transcription factor were also predicted and analyzed. Results showed that the disordered residues of CsDREB-A4 factor were obvious. The three-dimension structure of CsDREB-A4 factor was similar to AtERF1. The CsDREB-A4 factor, the majority of amino acid were hydrophilic, belonged to A4 group of DREB subfamily of AP2/ERF family transcription factors. The three-dimension structure was similar to AtERF1, the CsDREB-A4 gene was induced by high temperature and low temperature treatment in ‘Anjibaicha’, ‘Yingshuang’ and ‘Yunnanshilixiang’, respectively. The expression levels of CsDREB-A4 gene reached a maximum at 24 h in the three tea plant cultivars, which increased 23, 4, 43 times when exposed at 4℃. In ‘Yingshuang’ and ‘Yunnanshilixiang’, the expression levels of CsDREB-A4 gene were up-regulated more longer time and higher than that in ‘Anjibaicha’. The expression level of CsDREB-A4 gene was inhibited in ‘Yingshuang’ and ‘Yunnanshilixiang’ when exposed at 38℃ temperature, except at 8 h. In ‘Anjibaicha’, the expression level of CsDREB-A4 gene was increased obviously, and reached 2 720 times in 12 h than that in wild type tea plant.

Key words: Camellia sinensis, transcription factors, DREB subfamily, phylogenetic analysis, temperature stress, expression profile analysis

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