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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (3): 312-322.doi: 10.13305/j.cnki.jts.2016.03.011

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Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis

LI Hui1,3, HUANG Wei2, LIU Zhiwei1,3, WANG Yongxin1,3, WU Zhijun1,3, ZHUANG Jing1,3,*   

  1. 1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
    2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
    3. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2015-08-04 Online:2016-06-15 Published:2019-08-23

Abstract: Tea is an important economic crop. Tea production is affected by temperature factors. CsDof1 and CsDof2, which encode Dof transcription factors, were cloned from tea cultivar ‘Yingshuang’ [Camellia sinensis (L.) O. Kuntze]. The nucleotide and amino acid sequences, phylogenetic tree and molecular models of the CsDof1 and CsDof2 were analyzed. The results showed that the CsDof1 and CsDof2 are 1389 and 1458 nucleotides in length, which encode 463 and 486 amino acids respectively. Typical zinc finger domains are present in both CsDof1 and CsDof2, from coden 133 to 195 and 124 to 186 respectively. The CsDof1 and CsDof2 were hydrophilic proteins. The three-dimensional structures of CsDof1 and CsDof2 indicated the presence of a highly conserved Dof domain with four cysteine residues. Quantitative real-time PCR analysis showed that CsDof1 and CsDof2 were induced by different temperature stresses and the expression profiles were different among tea cultivars.

Key words: Camellia sinensis, Dof transcription factor, zinc finger, temperature stress, gene expression

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