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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (4): 363-371.doi: 10.13305/j.cnki.jts.2016.04.004

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Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro

ZHANG Jing1, CAI Shuxian2, HUANG Jian'an1,3,4, LI Juan1,3,4, ZHONG Ni1, YANG Heng1, LIU Zhonghua1,3,4,*   

  1. 1. Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, China;
    2. Peking University Health Science Center, Beijing 100191, China;
    3. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    4. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China
  • Received:2016-02-28 Published:2019-08-26

Abstract: A model of age pigment generation was builted by carbonyl-ammonia cross linking reaction in vitro, the datas indicate that theaflavins can not only inhibit the generation of age pigment in protein carbonylation, but also supress β-sheet-rich in aggregate protein in this model by thioflavin T-based fluorometric assay, scanning electron microscope, transmission electric microscope, SDS-PAGE and NBT staining, there are postive relationship between protein carbonylation on mechanism of aging and protein aggregation that lead to neurodegenerative disease.

Key words: theaflavin, carbonyl-ammonia cross linking reaction, age pigment, protein aggregation

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