Inhibitory Effect and Mechanism of Theaflavin (TF1) on Hepatoma Carcinoma Bel-7402 Cells

WANG Zhuan; WEI Wujun; REN Zhenzhen; HE Zhilong; FAN Yuchun; ZHOU Jie; GUO Guiyi; JIANG Lihe

doi:10.13305/j.cnki.jts.20230418.001

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (2) : 287-296. DOI: 10.13305/j.cnki.jts.20230418.001

Research Paper

Inhibitory Effect and Mechanism of Theaflavin (TF₁) on Hepatoma Carcinoma Bel-7402 Cells

WANG Zhuan¹, WEI Wujun², REN Zhenzhen², HE Zhilong¹, FAN Yuchun³, ZHOU Jie³, GUO Guiyi⁴, JIANG Lihe^1,2,3,*

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Abstract

To explore the inhibitory effect and mechanism of theaflavin (TF₁), human hepatoma carcinoma Bel-7402 cells were treated with different concentrations of TF₁. Cell viability was detected by CCK8 and cell proliferation was detected by colony formation assay. Cell migration was detected by cell wound healing experiment and transwell chamber assay. Cell apoptosis was detected by flow cytometry. Apoptosis-related proteins (Bax, Bcl-2, PARP), migration related proteins (E-cad, N-cad, Vimentin, MMP9) and signaling pathway related proteins (TGF-β, Smad3, p-smad3) were detected by western blot. The results show that different concentrations of TF₁ could inhibit the proliferation and migration of Bel-7402 cells and promote their apoptosis in a dose-dependent manner. The higher the dose, the stronger the inhibition effect (P<0.05). Compared with the control group, the expression levels of Bax and E-cad proteins in the experimental group were higher, while the expression levels of Bcl-2, PARP, N-cad, Vimentin, MMP9, TGF-β, Smad3 and p-smad3 were lower (P<0.05). In conclusion, TF₁ could inhibit the proliferation and migration of Bel-7402 cells and promote their apoptosis through TGF-β signaling pathway.

Key words

human hepatoma carcinoma cell / mechanism research / theaflavin

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WANG Zhuan, WEI Wujun, REN Zhenzhen, HE Zhilong, FAN Yuchun, ZHOU Jie, GUO Guiyi, JIANG Lihe. Inhibitory Effect and Mechanism of Theaflavin (TF₁) on Hepatoma Carcinoma Bel-7402 Cells[J]. Journal of Tea Science. 2023, 43(2): 287-296 https://doi.org/10.13305/j.cnki.jts.20230418.001

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