Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)

ZHENG Shizhong; JIANG Shengtao; LIU Wei; CHEN Meixia; LIN Yuling; LAI Zhongxiong; LIN Jinke

doi:10.13305/j.cnki.jts.2018.06.004

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (6) : 580-588. DOI: 10.13305/j.cnki.jts.2018.06.004

Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)

ZHENG Shizhong^1,2, JIANG Shengtao^1,2, LIU Wei^1,2, CHEN Meixia^1,2, LIN Yuling³, LAI Zhongxiong^3,*, LIN Jinke^4,*

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Abstract

The 1β828βbp full-length gDNA of CsMYB and a 1β038βbp 5′-flanking sequence of CsMYB (named proMYB) were cloned from the shoots of tea cultivar ‘1005’by gDNA cloning and genome-walking method. Bioinformatics analysis showed that the gDNA of CsMYB contained 2 introns and 3 extrons. The cis-acting element analysis showed that the proMYB promoter contained 5′-UTR Py-rich stretch motifs, TATA-box, CAAT-box core elements putative elements responding to hormone, light, stresses and some cis-elements with unknown or specific function, suggesting the inducible character of proMYB. It was also implied that CsMYB might be involved in abiotic stress responses and hormonal signal transduction in tea plant. The transient expression in transformed tobacco showed that the proMYB could drive the expressions of downstream genes.

Key words

CsMYB / Camellia sinensis / Cis-acting element / promoter / transient expression

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ZHENG Shizhong, JIANG Shengtao, LIU Wei, CHEN Meixia, LIN Yuling, LAI Zhongxiong, LIN Jinke. Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.)[J]. Journal of Tea Science. 2018, 38(6): 580-588 https://doi.org/10.13305/j.cnki.jts.2018.06.004

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