茶红素分离及稳定性研究

Abstract

Abstract: In this study, thearubigins were extracted by organic extraction, and purified by reversed-phase C₁₈column chromatography to obtain three types of thearubigins, namely ethyl acetate, n-butanol and acid n-butanol extracts. The stability of thearubigins under different conditions was analyzed by scanning UV-Vis absorption wavelength based on color difference. The results showed that there was no caffeine and theaflavins, but only a small amount of catechins were detected in the three kinds of thearubigins after 30%, 50% and 70% methanol gradient elution. The color values of thearubigins after purification were 128.04 for ethyl acetate extracts, 91.04 for n-butanol extracts and 76.16 for acidic n-butanol extracts. Shading treatment, pH=3 acidic conditions and temperature below 45℃will facilitate the stability of thearubigins.

Key words: thearubigins, chromatography, color values, stability

CLC Number:

LI Shan, CHEN Xiaoqiang, HUA Jinjie, LI Jia, DONG Chunwang, WANG Yincheng, JIANG Yongwen, YUAN Haibo. Separation and Stability Analysis of Thearubigins[J]. Journal of Tea Science, 2017, 37(2): 201-210.

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Separation and Stability Analysis of Thearubigins

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