超高效液相色谱-静电场轨道阱高分辨质谱法测定茶叶中21种农药残留量

doi:10.13305/j.cnki.jts.2018.06.006

Abstract

Abstract: A ultra performance liquid chromatography orbitrap high resolution mass spectrometry method for the determination of 21 pesticides in tea was developed. The tea samples were extracted by methanol-water (V_methanol∶V_water=1∶1), and cleaned up by a mixture of adsorbents containing primary secondary amine and strong cation exchanger. The chromatography analysis was performed on a C₁₈column. The target analytes were analyzed by high resolution mass spectrometry under Full Scan mode. The results showed that the linearities of 21 pesticides ranged from 0.5βμg·L^-1 to 200βμg·L^-1 with correlation coefficients higher than 0.99. At the spiked levels of 10, 50 and 100βμg·kg^-1, the average recoveries were from 70% to 125%, with the relative standard deviations (RSDs) below 15%. The limits of quantitation were 10βμg·kg^-1. The method is simple, rapid, sensitive and appropriate for the determination of 21 pesticide residues in tea.

Key words: tea, pesticide residues, ultra performance liquid chromatography orbitrap high resolution mass spectrometry

CLC Number:

TS272
O657.7

JIN Lili, HAO Zhenxia, GAO Guanwei, CHAI Yunfeng, WANG Chen, CHEN Hongping, LU Chengyin. Determination of 21 Pesticide Residues in Tea by Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry[J]. Journal of Tea Science, 2018, 38(6): 595-605.

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Determination of 21 Pesticide Residues in Tea by Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry

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