Determination of 21 Pesticide Residues in Tea by Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry

JIN Lili; HAO Zhenxia; GAO Guanwei; CHAI Yunfeng; WANG Chen; CHEN Hongping; LU Chengyin

doi:10.13305/j.cnki.jts.2018.06.006

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (6) : 595-605. DOI: 10.13305/j.cnki.jts.2018.06.006

Determination of 21 Pesticide Residues in Tea by Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry

JIN Lili^1,2, HAO Zhenxia¹, GAO Guanwei¹, CHAI Yunfeng¹, WANG Chen¹, CHEN Hongping¹, LU Chengyin^1,*

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Abstract

A ultra performance liquid chromatography orbitrap high resolution mass spectrometry method for the determination of 21 pesticides in tea was developed. The tea samples were extracted by methanol-water (V_methanol∶V_water=1∶1), and cleaned up by a mixture of adsorbents containing primary secondary amine and strong cation exchanger. The chromatography analysis was performed on a C₁₈column. The target analytes were analyzed by high resolution mass spectrometry under Full Scan mode. The results showed that the linearities of 21 pesticides ranged from 0.5βμg·L^-1 to 200βμg·L^-1 with correlation coefficients higher than 0.99. At the spiked levels of 10, 50 and 100βμg·kg^-1, the average recoveries were from 70% to 125%, with the relative standard deviations (RSDs) below 15%. The limits of quantitation were 10βμg·kg^-1. The method is simple, rapid, sensitive and appropriate for the determination of 21 pesticide residues in tea.

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pesticide residues / tea / ultra performance liquid chromatography orbitrap high resolution mass spectrometry

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JIN Lili, HAO Zhenxia, GAO Guanwei, CHAI Yunfeng, WANG Chen, CHEN Hongping, LU Chengyin. Determination of 21 Pesticide Residues in Tea by Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry[J]. Journal of Tea Science. 2018, 38(6): 595-605 https://doi.org/10.13305/j.cnki.jts.2018.06.006

References

[1] 农业部卫生和计划生育委员会. GB2763—2016食品安全国家标准食品中最大农药残留限量[S]. 北京: 中国标准出版社, 2016.
[2] 刘腾飞, 杨代凤, 张丽, 等. 茶叶中农药残留分析技术研究进展[J]. 食品科学技术学报, 2017, 35(4): 80-94.
[3] 陈红平, 刘新, 汪庆华, 等. 茶叶中7种除草剂农药残留气相色谱-串联质谱测定[J]. 茶叶科学, 2011, 31(4): 283-288.
[4] 农业部卫生和计划生育委员会. GB/T23204—2008茶叶中519种农药及相关化学品残留量的测定气相色谱-质谱法[S]. 北京: 中国标准出版社, 2009.
[5] 农业部卫生和计划生育委员会. GB23200.13—2016食品安全国家标准茶叶中448种农药及相关化学品残留量的测定液相色谱-质谱法[S]. 北京: 中国标准出版社, 2016.
[6] Kaufmann A, Butcher P, Maden K, et al.Comprehensive comparison of liquid chromatography selectivity as provided by two types of liquid chromatography detectors (high resolution mass spectrometry and tandem mass spectrometry): “Where is the crossover point?”[J]. Analytica Chimica Acta, 2010, 673(1): 60-72.
[7] 杨永坛, 陈士恒, 史晓梅. 食品中农药残留筛查系统的构建[J]. 食品安全质量检测学报, 2015, 6(4): 1101-1106.
[8] 熊岑, 李苑雯, 郑彦婕, 等. 静电场轨道阱质谱分析技术在食品分析中的应用进展[J]. 食品科学, 2015, 36(13): 283-287.
[9] 陈达炜, 高洁, 吕冰, 等. 超高效液相色谱-高分辨质谱法快速筛查土豆中的多种农药残留[J]. 分析化学, 2014, 42(4): 579-584.
[10] 吴斌, 丁涛, 柳菡, 等. 高效液相色谱-四极杆/静电场轨道阱高分辨质谱快速检测6种农产品中96种农药的残留量[J]. 色谱, 2012, 30(12): 1246-1252.
[11] 邱楠楠, 董桂贤, 陈达炜, 等. 自组装管尖固相萃取/超高效液相色谱-高分辨质谱法测定水果中的15种酸性农药[J]. 分析测试学报, 2017, 36(1): 31-36.
[12] 叶江雷, 金贵娥, 吴云辉, 等. QuEChERS法提取净化结合气-质联法快速检测茶叶中农药残留[J]. 食品科学, 2013, 34(12): 265-271.
[13] 赵暮雨, 韩芳, 孙锦文, 等. 多壁碳纳米管作为吸附剂的QuEChERS-气相色谱-四极杆飞行时间质谱快速筛查淡水产品中145种农药残留[J]. 分析测试学报, 2016, 35(12): 1513-1520.
[14] 刘妍慧, 于常红, 刘岩, 等. 基质固相分散萃取-高效液相色谱串联质谱法(HPLC-MS-MS)同时测定茶叶中11种农药的残留量[J].茶叶科学, 2014, 24(3): 271-278.
[15] 宋淑玲, 李重九, 马晓东, 等. 蔬菜中残留农药的石墨化碳黑净化和气相色谱-质谱检测方法[J]. 分析化学, 2008, 36(11): 1526-1530.
[16] González-Curbelo M Á, Socas-Rodríguez B, Herrera-Herrera A V, et al. Evolution and applications of the QuEChERS method[J]. TrAC Trends in Analytical Chemistry, 2015, 71: 169-185.
[17] 郭家刚. 茶叶中24种农药残留UPLC-MS/MS分析方法研究[D]. 合肥: 安徽农业大学, 2016.
[18] Chang J, Wang J, Chow W, et al.Application of ultrahigh-performance liquid chromatography and electrospray ionization quadrupole orbitrap high-resolution mass spectrometry for determination of 166 pesticides in fruits and vegetables[J]. Journal of Agricultural and Food Chemistry. 2012, 60(49): 12088-12104.
[19] Cladière M, Delaporte G, Le Roux E, et al.Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea[J]. Food Chemistry, 2018, 242: 113-121.
[20] European Commission. Analytical quality control and method validation procedures for pesticides residues analysis in food and feed, Document No.SANTE/11945/2015 [EB/OL]. 2016-01-01(2018-03-01). http://www.eurl-pesticides.eu/library/ docs/allcrl/ AqcGuidance_Sante_2015_11945.pdf.