Cloning and Expression Analysis of Na+/H+ Antiporter Gene CsNHX1 and CsNHX2 in Tea Plant (Camellia sinensis)

CHEN Jiangfei; YU Jinming; YANG Jiankun; YU Youben; XIAO Bin; YANG Yajun; WANG Weidong

doi:10.13305/j.cnki.jts.2018.06.002

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (6) : 559-568. DOI: 10.13305/j.cnki.jts.2018.06.002

Cloning and Expression Analysis of Na⁺/H⁺ Antiporter Gene CsNHX1 and CsNHX2 in Tea Plant (Camellia sinensis)

CHEN Jiangfei¹, YU Jinming¹, YANG Jiankun¹, YU Youben¹, XIAO Bin¹, YANG Yajun^1,2, WANG Weidong^1,*

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Abstract

The Na⁺/H⁺ antiporter (NHX) plays an important role in plant growth, development and stress response. In this study, the full-length cDNA sequences of CsNHX1 and CsNHX2 (GenBank: MG722977 and MG515211) were cloned from tea cultivar ‘Longjingchangye’. Bioinformatics analysis showed that the full-length cDNA of CsNHX1 and CsNHX2 were 1β691βbp and 1β757βbp, all containing a 1β626βbp open reading frame and encoding 541 amino acids. The molecular weights of CsNHX1 and CsNHX2 were 59.5βkD and 59.7βkD and pI were 7.07 and 8.79, respectively.The results of protein sequence analysis showed that CsNHX1 and CsNHX2 contained the conserved Na⁺/H⁺ exchange domain, and belong to the typical transmembrane proteins. Phylogenetic analysis of plant NHX revealed that CsNHX1 and CsNHX2 are the member of Class I that localized to the vacuolar membrane in IC subfamily. In addition, qRT-PCR results showed that the expressions of CsNHX1 and CsNHX2 were induced by drought, low-temperature and salt stress. In contrast, exogenous ABA could not induce the expressions of CsNHX1 and CsNHX2. In addition, the expression level of CsNHX1 in tea plant decreased significantly, but that of CsNHX2 increased gradually under heat stress, indicating that CsNHX1 and CsNHX2 were differently involved in tea plant responding to environmental stress, and possibly through different responding modes.

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expression analysis / gene cloning / NHX / tea plant

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CHEN Jiangfei, YU Jinming, YANG Jiankun, YU Youben, XIAO Bin, YANG Yajun, WANG Weidong. Cloning and Expression Analysis of Na⁺/H⁺ Antiporter Gene CsNHX1 and CsNHX2 in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2018, 38(6): 559-568 https://doi.org/10.13305/j.cnki.jts.2018.06.002

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