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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (3): 157-165.doi: 10.13305/j.cnki.jts.2008.03.003

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A Sequence Analysis and Structural Prediction on Polyphenol Oxidase in Tea

PAN Yu1,2, WANG Kun-bo1,2, XU Zhong-xi1,2,*, XU Hui3, HE Zhi-dan1,2   

  1. 1. The Key Laboratory of Tea Science of National Ministry of Education, Hunan Agricultural University
    2. Horticulture and Landscape College, Hunan Agricultural University
    3. Biological Sciences & Technology College, Hunan Agricultural University, Changsha 410128, China
  • Received:2007-11-26 Revised:2008-03-06 Online:2008-06-15 Published:2019-09-16

Abstract: In the present study, Polyphenol Oxidase (PPO) in tea plant which was registered in biodatabase (DDBJ & Uni-Prot) were analyzed and predicted by method of bioinformatics, including composition of nuclei acid sequences and amino acid sequences, signal peptide, transmembrane topological structure, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein. The results showed that PPO in tea was a hydrophilic and non-transmembrane protein without signal peptide, but had coils. The main motifs of its predicted secondary structure of had random coil, alpha helix and beta turn were spread to the whole secondary structure of protein. Its amino acid sequences included two functional domains.

Key words: tea plant, polyphenol oxidase, sequence analysis, structural prediction

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