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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (1): 58-68.doi: 10.13305/j.cnki.jts.2018.01.006

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Analysis of Genetic Diversity and Construction of DNA Fingerprints of Chlorophyll-deficient Tea Cultivars by SSR Markers

WANG Songlin1,2, MA Chunlei1,2, HUANG Danjuan1, MA Jianqiang1, JIN Jiqiang1, YAO Mingzhe1,*, CHEN Liang1,*   

  1. 1. Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agriculture Science, Beijing 100081, China
  • Received:2017-07-07 Revised:2017-08-21 Online:2018-02-15 Published:2019-08-28

Abstract: To differentiate and identify different albino tea cultivars, sixty two SSR primers were used to analyze the genetic diversity of 16 tea cultivars that exhibit the chlorina phenotype. The result showed that a total of 169 alleles were amplified by 55 SSR primers with good polymorphism, and the number of alleles per primer ranged from 2 to 5, with an average of 3.07. The average value of polymorphism information content (PIC) and Shannon’s information information index (I) was 0.40 and 0.79 respectively, which indicate these albino and yellow-leaf tea cultivars having a moderate level of diversity. And the occurrence frequency of 169 alleles ranged from 3.12% to 96.88%. It suggested the difference of genetic structure among tested varieties is obvious. The Nei's genetic distance (D) of sixteen tested cultivars ranged from 0.086 to 0.532. These cultivars could be divided into three categories when D was 0.18, and the cultivars with close relatives or similar geographical origin were clustered into one group. Lastly, three primers (TM156, TM508, MSG0380) with easy identification, good stability and high polymorphism, were finally chosen to establish the fingerprint. The 16 albino and yellow-leaf tea cultivars could be effectively distinguished by the primers. This study provided an important basis for the identification of albino tea cultivars, and the evaluation and utilization of these germplasm resources.

Key words: albino tea cultivars, SSR marker, fingerprinting

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