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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (3): 261-268.doi: 10.13305/j.cnki.jts.2012.03.011

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Analysis of Genetic Diversity and Construction of DNA Fingerprinting with EST-SSR Markers for Improved Clonal Tea Cultivars in Yunnan Province

LIU Ben-ying1, SUN Xue-mei1, LI You-yong1, HUANG An-ping2, WANGYun-gang1, CHENG Hao3, SONG Wei-xi1, CHEN Lin-bo1, DUAN Zhi-fen1, MA Ling1   

  1. 1. Tea Research Institute, Yunnan Academy of Agricultural Science, Menghai 666201, China;
    2. Tea Research Institute, Hunan Academy of Agricultural Science, Changsha 410125, China;
    3. Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China
  • Received:2012-02-02 Revised:2012-03-07 Online:2012-06-15 Published:2019-09-05

Abstract: Analysis of genetic diversity and construction of molecular fingerprints is very important to breed new tea cultivars and identify new varieties, cultivars authenticity and seedings purity. Twenty-two EST-SSR primer pairs with better polymorphism in this study were used to research the genetic diversity and DNA fingerprinting of 28 tea cultivars. A total of 56 putative alleles were generated with a mean of 2.55 for putative alleles per locus. Totally 97 genotypes were detected in all materials with a mean of 4.41 for each polymorphism primer pairs. The polymorphism information content (PIC) varied from 0.279 to 0.709, with a higher average of 0.527. These results showed a high level polymorphism in SSR markers. The genetic similarity coefficient among 28 tea cultivars ranged from 0.642 to 0.973 with an average of 0.797, suggesting that the tea cultivars used in the experiment possessed a low level polymorphism and a narrow genetic variation. The EST-SSR alleles were determined for bands size (bp) and scored as present (1) or absent (0). According to the genotypes code rule, the 1 and 0 results which one pair of SSR primers amplified in a cultivar could be converted to genotypes code following their fragment size. The results proved that it is a very useful tool for discriminating tea cultivar, and all accessions could be discriminated from a set of molecular fingerprints.

Key words: EST-SSR marker, improved clonal tea cultivars, genetic diversity, DNA fingerprinting

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