Genetic Diversity Estimates for Darjeeling Tea Clones Based on Amplified Fragment Length Polymorphism Markers

Rajan Kumar Mishra; Swati Sen-Mandi

doi:10.13305/j.cnki.jts.2004.02.003

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Journal of Tea Science ›› 2004, Vol. 24 ›› Issue (2) : 86-92. DOI: 10.13305/j.cnki.jts.2004.02.003

Genetic Diversity Estimates for Darjeeling Tea Clones Based on Amplified Fragment Length Polymorphism Markers

Rajan Kumar Mishra, Swati Sen-Mandi

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Abstract

Tea plants growing in Darjeeling area, in addition to all other factors described below, are unique due to their typical geographical isolation. DNA fingerprints revealed a high degree of polymorphism in genomes of different clones, and this demonstrated the suitability of using the Amplified Fragment Length Polymorphism (AFLP) method for genome analysis between closely related plants, such as vegetatively propagated (clonal) populations of tea. Cluster analysis exhibited a dendrogram that closely matched with earlier clonal grouping based on morphological characters. The extent of genetic relatedness between the clones was found to be at 70% level. Results also showed that the genetic variation (Hs) was higher among China type. The variation between and within the three types of clonal populations studied (viz. China, Assam and Cambod type) are 63% and 36% respectively.

Key words

Camellia sinensis / DNA fingerprinting / Genetic diversity / Shannon’s diversity index / AFLP

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Rajan Kumar Mishra, Swati Sen-Mandi. Genetic Diversity Estimates for Darjeeling Tea Clones Based on Amplified Fragment Length Polymorphism Markers[J]. Journal of Tea Science. 2004, 24(2): 86-92 https://doi.org/10.13305/j.cnki.jts.2004.02.003

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