顶空气相色谱法测定绿茶提取物中乙酸乙酯残留量

doi:10.13305/j.cnki.jts.2007.01.010

茶叶科学 ›› 2007, Vol. 27 ›› Issue (1): 67-70.doi: 10.13305/j.cnki.jts.2007.01.010

顶空气相色谱法测定绿茶提取物中乙酸乙酯残留量

胡慧廉, 丁乃立, 吴莲珍

华东理工大学分析测试中心,上海 200237

收稿日期:2006-09-13 修回日期:2006-12-13 出版日期:2007-03-25 发布日期:2019-09-11
作者简介:胡慧廉(1963— ),女,工程师,硕士,从事色谱分析研究。E-mail: huhl@ecust.edu.cn
基金资助:
上海市自然科学基金（05ZR14088）; 上海市重点学科建设项目（T0503）,上海市联盟计划–难题攻关项目

Determination of Residual Ethyl Acetate in Green Tea Extract by Headspace Gas Chromatography

HU Hui-lian, DING Nai-li, WU Lian-zhen

Analysis and Research Center, East China University of Science and Technology, Shanghai 200237, China

Received:2006-09-13 Revised:2006-12-13 Online:2007-03-25 Published:2019-09-11

摘要/Abstract

摘要： 采用顶空气相色谱法对绿茶提取物中乙酸乙酯残留溶剂的分析方法进行了研究。试验采用大口径HP-快速GC残留溶剂柱为分离柱,对顶空平衡温度、平衡时间对残留有机溶剂乙酸乙酯测定的影响进行了分析研究。结果表明：乙酸乙酯在0.0765~0.612μg/ml内线性关系良好,相关系数为0.9993;方法精密度试验RSD(n=6)为3.60%;平均回收率范围95.42%~97.88%,RSD 1.95%~3.09%;检测限(S/N=3)为0.005μg/ml。本方法快速、灵敏、准确、无污染,适用于绿茶提取物中乙酸乙酯残留量的测定。

关键词: 顶空气相色谱法, 绿茶提取物, 乙酸乙酯, 残留有机溶剂

Abstract: A method for the determination of residual ethyl acetate in green tea extract has been studied by headspace gas chromatography in order to set up an analysis method. A wide bore HP-fast GC residual solvent column was performed, and the effects of the equilibrium temperature and equilibrium time on the determination of ethyl acetate were studied. The results showed that the standard curve was linear in the range of 0.0765~0.612μg/ml (r=0.9993). the relative standard deviation (n=6) of precision was 3.60%. the average recoveries were between 95.42% and 97.88% with RSD in the range of 1.95%~3.09%, the minimum detectable concentration(S/N=3) was 0.005μg/ml. It is a rapid, sensitive, accurate and uncontaminated method and applicable for the determination of residual ethyl acetate in green tea extract.

Key words: headspace gas chromatography, green tea extract, ethyl acetate, residual organic solvent

中图分类号:

胡慧廉, 丁乃立, 吴莲珍. 顶空气相色谱法测定绿茶提取物中乙酸乙酯残留量[J]. 茶叶科学, 2007, 27(1): 67-70. doi: 10.13305/j.cnki.jts.2007.01.010.

HU Hui-lian, DING Nai-li, WU Lian-zhen. Determination of Residual Ethyl Acetate in Green Tea Extract by Headspace Gas Chromatography[J]. Journal of Tea Science, 2007, 27(1): 67-70. doi: 10.13305/j.cnki.jts.2007.01.010.

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顶空气相色谱法测定绿茶提取物中乙酸乙酯残留量

Determination of Residual Ethyl Acetate in Green Tea Extract by Headspace Gas Chromatography

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