茶尺蠖为害提高邻近茶苗对茶尺蠖幼虫的防御能力

雷舒; 李喜旺; 孙晓玲; 王志英; 辛肇军

doi:10.13305/j.cnki.jts.2016.06.005

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茶叶科学 ›› 2016, Vol. 36 ›› Issue (6) : 587-593. DOI: 10.13305/j.cnki.jts.2016.06.005

茶尺蠖为害提高邻近茶苗对茶尺蠖幼虫的防御能力

雷舒^1,2, 李喜旺^2,3, 孙晓玲^2,3, 王志英^1,*, 辛肇军^2,3,*

作者信息 +

Infestation of Ectropis obliqua Enhances Neighboring Tea Plant Defenses Against Conspecific Larvae

LEI Shu^1,2, LI Xiwang^2,3, SUN Xiaoling^2,3, WANG Zhiying^1,*, XIN Zhaojun^2,3,*

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摘要

本文研究了茶树虫害诱导挥发物（Herbivore-induced plant volatiles, HIPVs）对邻近茶苗防御能力的影响。将健康茶苗放在茶尺蠖取食的茶苗附近,作为HIPVs的处理,测定了茶树防御相关基因在不同处理下的表达水平、抗性防御物质的水平,并检测了茶尺蠖危害茶苗邻近植株对茶尺蠖幼虫生长的影响。研究结果表明,与对照相比,茶尺蠖幼虫为害茶苗释放的HIPVs在处理后24 h和12 h内分别显著诱导邻近茶苗CsiLOX1和CsiACS1基因的表达水平,这些挥发物还能增强茶尺蠖取食诱导的防御基因的表达。HIPVs处理3 d后,茶树的重要防御物质多酚氧化酶（Polyphenol oxidases, PPOs）活性显著高于对照,是对照的1.36倍,同时,取食这些茶苗的茶尺蠖幼虫体重也显著低于对照。上述结论表明茶尺蠖取食诱导的挥发物能够作为信号物质在茶树中传递,并能够通过增强茉莉酸和乙烯抗虫途径提高对茶尺蠖的抗性。

Abstract

In this study, the effect of herbivore-induced plant volatiles (HIPVs) from tea plant on the defense responses of neighboring tea plants was analyzed. The expression levels of defense-related genes, the content of defense compound in tea and the mass of Ectropis obliqua (TG) larvae were measured respectively. Compared with the control plants, the results showed that HIPVs released from TG-infested tea plants significantly induced the relative expression levels of CsiLOX1 and CsiACS1 in the neighboring tea plants within 24 h and 12 h. TG-induced expression of CsiLOX1 and CsiACS1 were also enhanced by HIPVs. Three days after HIPVs exposure, the polyphenol oxidases（PPOs） activity in the treated tea leaves was 1.36-fold higher than that of control. Meanwhile, the HIPVs-treated tea plants reduced the performance of TG. The above results showed that volatiles released from TG-infested tea can convey information to those neighboring plants and then trigger the induced defense responses against TG by modulating JA and ET signaling pathways in tea.

导出引用

雷舒, 李喜旺, 孙晓玲, 王志英, 辛肇军. 茶尺蠖为害提高邻近茶苗对茶尺蠖幼虫的防御能力[J]. 茶叶科学. 2016, 36(6): 587-593 https://doi.org/10.13305/j.cnki.jts.2016.06.005

LEI Shu, LI Xiwang, SUN Xiaoling, WANG Zhiying, XIN Zhaojun. Infestation of Ectropis obliqua Enhances Neighboring Tea Plant Defenses Against Conspecific Larvae[J]. Journal of Tea Science. 2016, 36(6): 587-593 https://doi.org/10.13305/j.cnki.jts.2016.06.005

中图分类号： S571.1 S435.711

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