茶尺蠖幼虫取食提高茶树儿茶素代谢响应强度

冉伟; 张瑾; 张新; 蔺松波; 孙晓玲

doi:10.13305/j.cnki.jts.2018.02.003

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茶叶科学 ›› 2018, Vol. 38 ›› Issue (2) : 133-139. DOI: 10.13305/j.cnki.jts.2018.02.003

茶尺蠖幼虫取食提高茶树儿茶素代谢响应强度

冉伟^1,2, 张瑾^1,2, 张新^1,2, 蔺松波^1,2, 孙晓玲^1,2,*

作者信息 +

Infestation of Ectropis obliqua Affects the Catechin Metabolism in Tea Plants

RAN Wei^1,2, ZHANG Jin^1,2, ZHANG Xin^1,2, LIN Songbo^1,2, SUN Xiaoling^1,2,*

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文章历史 +

摘要

研究了茶尺蠖幼虫为害茶树叶片对儿茶素合成途径的影响。采用3龄茶尺蠖幼虫取食茶树新梢芽下第二叶,测定了儿茶素合成相关基因的表达水平和儿茶素含量。研究结果表明,与对照相比,茶尺蠖幼虫为害后3、6、12βh显著诱导了CsANR基因的相对表达水平,且在为害后3βh和12βh达到了极显著差异。CsLAR基因在茶尺蠖为害后6βh和12βh,与对照具有显著差异。茶尺蠖幼虫为害后24βh显著诱导了没食子酸、没食子儿茶素、表儿茶素、表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯含量的升高,而茶尺蠖为害后48βh仅没食子酸和没食子儿茶素的含量显著高于对照;儿茶素、表没食子儿茶素和没食子儿茶素没食子酸酯在茶尺蠖为害后24βh和48βh均没有被显著诱导。上述结果表明茶尺蠖幼虫为害提高了茶树儿茶素合成途径的代谢强度和儿茶素类化合物的积累。

Abstract

In this study, the effect of feeding by Ectropis obliqua on the catechin pathway of tea plant was analyzed. The transcriptional levels of catechin-related genes and the contents of individual catechins in the infested or intact leaves were measured. The transcriptional level of CsANR in the infested leaves was significantly higher than that in the intact leaves 3, 6βh and 12βh after infestation. Meanwhile, the infestation of E. obliqua also significantly induced the expression level of CsLAR after 6βh and 12βh. The contents of gallic acid, gallocatechin, epicatechin, epigallocatechin gallate and epicatechin gallate were significantly induced 24βh after infestation. Moreover, the contents of gallic acid and gallocatechin were also significantly induced 48βh after infestation. However, the infestation of E. obliqua didn’t induce the increases of catechin, epigallocatechin and gallocatechin gallate. In a word, the infestation of E. obliqua affected catechin metabolism in tea plant.

导出引用

冉伟, 张瑾, 张新, 蔺松波, 孙晓玲. 茶尺蠖幼虫取食提高茶树儿茶素代谢响应强度[J]. 茶叶科学. 2018, 38(2): 133-139 https://doi.org/10.13305/j.cnki.jts.2018.02.003

RAN Wei, ZHANG Jin, ZHANG Xin, LIN Songbo, SUN Xiaoling. Infestation of Ectropis obliqua Affects the Catechin Metabolism in Tea Plants[J]. Journal of Tea Science. 2018, 38(2): 133-139 https://doi.org/10.13305/j.cnki.jts.2018.02.003

中图分类号： S571.1 S435.711

参考文献

[1] 宛晓春. 茶叶生物化学[M]. 3版. 北京: 中国农业出版社, 2003.
[2] Jiang X, Feng K, Yang X.In vitro antifungal activity and mechanism of action of tea polyphenols and tea saponin against Rhizopus stolonifera[J]. J Mol Microbiol Biotechnol, 2015, 35(7): 269-276.
[3] Mikulic-Petkovsek M, Schmitzer V, Jakopic J, et al.Phenolic compounds as defence response of pepper fruits to Colletotrichum coccodes [J]. Physiol Mol Plant Pathol, 2013, 84(1): 138-145.
[4] Yi S M, Zhu J L, Fu L L, et al.Tea polyphenols inhibit Pseudomonas aeruginosa through damage to the cell membrane[J]. Int J Food Microbiol, 2010, 144(1): 111-117.
[5] Aditi S, Kanwar S S, Sud R G, et al.Influence of phenolic compounds of Kangra tea [Camellia sinensis(L) O Kuntze] on bacterial pathogens and indigenous bacterial probiotics of Western Himalayas[J]. Braz J Microbiol. 2013, 44(3): 709-715.
[6] Wang Y C, Qian W J, Li N N, et al.Metabolic changes of caffeine in tea pant (Camellia sinensis (L.) O. Kuntze) as defense response to colletotrichum fructicola[J]. Journal of Agricultural & Food Chemistry, 2016, 64(35): 6685-6693.
[7] Siranidou E, Kang Z, Buchenauer H.Studies on symptom development, phenolic compounds and morphological defense responses in wheat cultivars differing in resistance to fusarium, head bight[J]. Journal of Phytopathology, 2002, 150(5): 200-208.
[8] Czerniewicz P, Sytykiewicz H, Durak R, et al.Role of phenolic compounds during antioxidative responses of winter triticale to aphid and beetle attack[J]. Plant Physiology & Biochemistry Ppb, 2017(118): 529-540.
[9] 郑高云. 不同茶树品种对茶尺蠖抗性机制的研究[D]. 合肥: 安徽农业大学, 2008.
[10] 金珊. 不同茶树品种抗假眼小绿叶蝉机理研究[D]. 杨凌: 西北农林科技大学, 2012.
[11] 高香凤, 李慧玲, 王庆森. 茶树叶片组织结构及次生物质与抗虫性关系研究进展[J]. 茶叶科学技术, 2011(2): 7-11.
[12] Mohanpuria P, Kumar V, Yadav S K.Tea caffeine: Metabolism, functions, and reduction strategies[J]. Food Science & Biotechnology, 2010, 19(2): 275-287.
[13] Xin Z, Zhang Z, Chen Z, et al.Salicylhydroxamic acid (SHAM) negatively mediates tea herbivore-induced direct and indrect defense against the tea geometrid Ectropis obliqua[J]. J Plant Res, 2014, 127(4): 565-572.
[14] Fragoso V, Rothe E, Baldwin I T, et al.Root jasmonic acid synthesis and perception regulate folivore-induced shoot metabolites and increase Nicotiana attenuata resistance[J]. New Phytologist, 2014, 202(4): 1335-1345.
[15] Sun X L, Wang G C, Gao Y, et al.Volatiles emitted from tea plants infested by Ectropis obliqua larvae are attractive to conspecifc moths[J]. Journal of Chemical Ecology, 2014, 40(10): 1080-1089.
[16] 孙晓玲, 高宇, 陈宗懋. 虫害诱导植物挥发物(HIPVs)对植食性昆虫的行为调控[J]. 应用昆虫学报, 2012, 49(6): 1413-1422.
[17] 雷舒, 李喜旺, 孙晓玲, 等. 茶尺蠖为害提高临近茶苗对茶尺蠖幼虫的防御能力[J]. 茶叶科学, 2016, 36(6): 587-593.
[18] 张琪, 徐维玲, 李翠芹. HPLC法同时测定茶叶中儿茶素类和咖啡因的含量[J]. 食品工业科技, 2015, 36(4): 53-56.
[19] Kessler A, Baldwin IT.Plant responses to insect herbivory: the emerging molecular hypothesis[J]. Annu Rev Plant Biol, 2002, 53(1): 299-328. DOI: 10.1146/annurev.arplant. 53.100301.135207.
[20] War AR, Paulraj MG, Hussain B, et al.Effect of plant secondary metabolites on legume pod borer, helicoverpa armigera[J]. Journal of Pest Science, 2013, 86(3): 399-408.
[21] Scogings P F, Hjältén J, Skarpe C, et al.Nutrient and secondary metabolite concentrations in a savanna are independently affected by large herbivores and shoot growth rate[J]. Plant Ecology, 2014, 215(1): 73-82.
[22] Lattanzio V, Lattanzio V M T, Cardinali A. Role of polyphenols in the resistance mechanisms of plants against fungal pathogens and insects[M]. Imperato, F. Phytochemistry: Advances in research, Research Signpost. Trivandrum, Kerala, India, 2006: 23-67.
[23] Wójcicka A.Cereal phenolic compounds as biopesticides of cereal aphids[J]. Polish Journal of Environmental Studies, 2010, 19(6): 1337-1343.
[24] 刘泽辉, 赵国虎, 陆敬善, 等. 棉花棉酚含量与抗虫特性的研究[J]. 新疆农业科学, 2008, 45(3): 409-413.
[25] Punyasiri P A, Abeysinghe I S, Kumar V, et al.Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways[J]. Archives of Biochemistry & Biophysics, 2004, 431(1): 22-30.
[26] Felton G, Donato K, Broadway R, et al.Impact of oxidized plant phenolics on the nutritional quality of dietar protein to a noctuid herbivore, Spodoptera exigua[J]. Journal of Insect Physiology, 1992, 38(4): 277-285
[27] Wang J, Constabel C P.Polyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria)[J]. Planta, 2004, 220(1): 87-96.
[28] Bhonwong A, Stout MJ, Attajarusit J, et al.Defensive role of tomato polyphenol oxidases against cotton bollworm (Helicoverpa armigera) and beet armyworm (Spodoptera exigua)[J]. Journal of Chemical Ecology, 2009, 35(1): 28-38.
[29] Vanitha S C, Umesha S.Role of phenylalanine ammonia lyase and polyphenol oxidase in host resistance to bacterial wilt of tomato[J]. Journal of Phytopathology, 2010, 157(9): 552-557.
[30] Bosch M, Berger S, Schaller A, et al.Jasmonate-dependent induction of polyphenol oxidase activity in tomato foliage is important for defense against Spodoptera exigua but not against Manduca sexta[J]. Bmc Plant Biology, 2014, 14(1): 257-272.
[31] 朱香镇, 麻巧迎, 张帅, 等. 棉花多酚氧化酶基因GhPPO1的克隆及在棉铃虫取食诱导反应中的作用[J]. 中国农业科学, 2014, 47(16): 3174-3183.
[32] Mishra BB, Gautam S.Polyphenol oxidases: biochemical and molecular characterization, distribution, role and its control[J]. Enz Eng, 2016, 5: 141. Doi:10.4172/2329-6674.1000141.
[33] Liang X, Chen Q, Lu H, et al.Increased activities of peroxidase and polyphenol oxidase enhance cassava resistance to Tetranychus urticae[J]. Experimental and Applied Acarology, 2017, 71(3), 195-209.
[34] Yang ZW, DUAN XN, Jin S, et al.Regurgitant derived from the tea geometrid ectropis obliqua suppresses, wound-induced polyphenol oxidases activity in tea plants[J]. Journal of Chemical Ecology, 2013, 39(6): 744-751.