不同贮藏年份政和白茶的香气特征与挥发性成分分析

黄维; 张灵枝; 张嘉琳; 林馥茗; 荣杰峰; 肖春燕; 岳鹏航; 余华珠; 孙威江; 黄艳

doi:10.13305/j.cnki.jts.2023.05.006

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茶叶科学 ›› 2023, Vol. 43 ›› Issue (5) : 667-680. DOI: 10.13305/j.cnki.jts.2023.05.006

研究报告

不同贮藏年份政和白茶的香气特征与挥发性成分分析

黄维^1,2, 张灵枝^1,2, 张嘉琳^1,2, 林馥茗^2,3, 荣杰峰⁴, 肖春燕^1,2, 岳鹏航³, 余华珠⁵, 孙威江^1,2,*, 黄艳^2,3,*

作者信息 +

Analysis of Aroma Characteristics and Volatile Components of Zhenghe White Tea with Different Storage Years

HUANG Wei^1,2, ZHANG Lingzhi^1,2, ZHANG Jialin^1,2, LIN Fuming^2,3, RONG Jiefeng⁴, XIAO Chunyan^1,2, YUE Penghang³, YU Huazhu⁵, SUN Weijiang^1,2,*, HUANG Yan^2,3,*

Author information +

文章历史 +

摘要

为探究不同贮藏年份政和白茶的香气特征,采用定量描述分析结合顶空固相微萃取-气相色谱-质谱联用技术,对贮藏0、5、10、15年的政和白牡丹白茶（分别编号BMD0、BMD5、BMD10和BMD15）的香气特征与挥发性成分进行分析。结果表明,不同贮藏年份政和白牡丹的香气特征差异明显,BMD0以毫香、清香、甜香、花香为主,随贮藏时间延长,毫香、清香、甜香、花香减弱,陈香、木香增强。不同贮藏年份的政和白牡丹共检测出66种挥发性成分,以醇类、酯类、酸类为主,BMD0和BMD5醇类含量较高,BMD10和BMD15酯类含量较高,随贮藏年份增加,挥发性成分总量显著降低,相对含量的组成与占比变化显著。所建立的正交偏最小二乘判别分析模型,可将不同贮藏年份政和白牡丹进行有效区分,分别以相对香气活性值（Relative odor activity value,rOAV）>1、变量投影重要性（Variable important for the projection,VIP）>1为依据,筛选出14种和30种差异挥发性成分;以rOAV>1且VIP>1为依据,筛选出β-紫罗兰酮、α-紫罗兰酮、反式-橙花叔醇、苯甲醇、苯乙醛、α-亚麻酸共6种关键差异挥发性成分。本研究为白茶科学储藏、陈年白茶风味解析提供理论参考与依据。

Abstract

In order to investigate the aroma characteristics of Zhenghe white tea with different storage years, quantitative descriptive analysis (QDA) combined with headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to analyze the aroma characteristics and volatile components of Zhenghe white peony tea stored for 0, 5, 10 and 15 years. The results show that the aroma characteristics of Zhenghe white peony tea varied significantly among different storage years. The aroma characteristics of BMD0 were mainly pekoe, fresh, sweet and floral. With the extension of storage time, the pekoe, fresh, sweet and floral weakened, while the stale flavour and woody increased. A total of 66 volatile components were detected in Zhenghe white peony tea with different storage years, mainly alcohols, esters and acids, with the highest alcohol content in BMD0 and BMD5, and the highest ester content in BMD10 and BMD15. With the extension of storage time, the total volatile components decreased significantly, and the composition and proportion of relative contents changed significantly. The Orthogonal partial least-squares discrimination analysis (OPLS-DA) model could effectively discriminate Zhenghe white peony tea with different storage years. A total of 14 volatile components were screened based on relative odor activity value (rOAV)>1, while 30 volatile components were screened based on variable importance for the projection (VIP)>1. Based on rOAV value>1 and VIP value>1, 6 major volatile components were screened, including β-ionone, α-ionone, nerolidol, benzyl alcohol, benzeneacetaldehyde, linolenic acid. This study provided a theoretical reference and basis for the scientific storage of white tea and the flavour analysis of aged white tea.

导出引用

黄维, 张灵枝, 张嘉琳, 林馥茗, 荣杰峰, 肖春燕, 岳鹏航, 余华珠, 孙威江, 黄艳. 不同贮藏年份政和白茶的香气特征与挥发性成分分析[J]. 茶叶科学. 2023, 43(5): 667-680 https://doi.org/10.13305/j.cnki.jts.2023.05.006

HUANG Wei, ZHANG Lingzhi, ZHANG Jialin, LIN Fuming, RONG Jiefeng, XIAO Chunyan, YUE Penghang, YU Huazhu, SUN Weijiang, HUANG Yan. Analysis of Aroma Characteristics and Volatile Components of Zhenghe White Tea with Different Storage Years[J]. Journal of Tea Science. 2023, 43(5): 667-680 https://doi.org/10.13305/j.cnki.jts.2023.05.006

中图分类号： S571.1

参考文献

[1] Sanlier N, Atik İ, Atik A.A minireview of effects of white tea consumption on diseases[J]. Trends in Food Science & Technology, 2018, 82: 82-88.
[2] Yang C, Hu Z Y, Lu M L, et al.Application of metabolomics profiling in the analysis of metabolites and taste quality in different subtypes of white tea[J]. Food Research International, 2018, 106: 909-919.
[3] 丁玎, 宁井铭, 张正竹, 等. 不同等级和储藏时间白茶香气组分差异性研究[J]. 安徽农业大学学报, 2016, 43(3): 337-344.
Ding D, Ning J M, Zhang Z Z, et al.Volatile compounds of white tea of different grades and different storage times[J]. Journal of Anhui Agricultural University, 2016, 43(3): 337-344.
[4] Chen Q C, Zhu Y, Yan H, et al.Identification of aroma composition and key odorants contributing to aroma characteristics of white teas[J]. Molecules, 2020, 25(24): 6050. doi: 10.3390/molecules25246050.
[5] Wang Z H, Wang Z H, Dai H M, et al.Identification of characteristic aroma and bacteria related to aroma evolution during long-term storage of compressed white tea[J]. Frontiers in Nutrition, 2022, 9: 1092048. doi: 10.3389/fnut.2022.1092048.
[6] 冯花, 王飞权, 张渤, 等. 不同茶树品种白牡丹茶香气成分的HS-SPME-GC-MS分析[J]. 现代食品科技, 2021, 37(12): 252-264.
Feng H, Wang F Q, Zhang B, et al.Analysis of aroma components of Baimudan tea from different tea plant varieties using HS-SPME-GC-MS[J]. Modern Food Science and Technology, 2021, 37(12): 252-264.
[7] Wang K B, Liu F, Liu Z H, et al.Comparison of catechins and volatile compounds among different types of tea using high performance liquid chromatograph and gas chromatograph mass spectrometer[J]. International Journal of Food Science and Technology, 2011, 46(7): 1406-1412.
[8] 陈维, 马成英, 王雯雯, 等. 萎凋时间对“英红九号”白茶香气的影响[J]. 食品科学, 2017, 38(18): 138-143.
Chen W, Ma C Y, Wang W W, et al.Effects of withering duration on the aroma profile of Yinghong No. 9 white tea[J]. Food Science, 2017, 38(18): 138-143.
[9] 石碧滢, 周承哲, 田采云, 等. 不同贮藏时间白牡丹茶风味品质差异分析[J]. 食品科学, 2023, 44(14): 313-325.
Shi B Y, Zhou C Z, Tian C Y, et al.Analysis of flavor quality differences between white peony tea with different storage time[J]. Food Science,2023, 44(14): 313-325.
[10] 周琼琼, 孙威江, 叶艳, 等. 不同年份白茶的主要生化成分分析[J]. 食品工业科技, 2014, 35(9): 351-354, 359.
Zhou Q Q, Sun W J, Ye Y, et al.Study on the main biochemical components of white tea stored at different years[J]. Science and Technology of Food Industry, 2014, 35(9): 351-354, 359.
[11] 丁玎. 不同等级和储藏时间白茶主要化学品质成分分析[D]. 合肥: 安徽农业大学, 2016.
Ding D.Analysis on chemical components of white tea with different grades and storage times[D]. Hefei: Anhui Agricultural University, 2016.
[12] 陈文杰. 福建省政和白茶品牌建设研究[D]. 福州: 福建农林大学, 2017.
Chen W J.Study on the brand construction of Zhenghe white tea in Fujian Province[D]. Fuzhou: Fujian Agriculture and Forestry University, 2017.
[13] Hong X, Wang C, Jiang R G, et al.Characterization of the key aroma compounds in different aroma types of Chinese yellow tea[J]. Foods, 2022, 12(1): 27. doi: 10.3390/foods12010027.
[14] Hao Z L, Feng J, Chen Q L, et al.Comparative volatiles profiling in milk-flavored white tea and traditional white tea Shoumei via HS-SPME-GC-TOFMS and OAV analyses[J]. Food Chemistry: X, 2023, 18: 100710. doi: 10.1016/j.fochx.
2023.100710.
[15] Dool H V D, Kratz P D. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography[J]. Journal of Chromatography A, 1963, 11: 463-471.
[16] Su D, He J J, Zhou Y Z, et al.Aroma effects of key volatile compounds in Keemun black tea at different grades: HS-SPME-GC-MS, sensory evaluation, and chemometrics[J]. Food Chemistry, 2022, 373(B): 131587. doi: 10.1016/j.foodchem.2021.131587.
[17] Deng X J, Huang G H, Tu Q, et al.Evolution analysis of flavor-active compounds during artificial fermentation of Pu-erh tea[J]. Food Chemistry, 2021, 357(2): 129783. doi: 10.1016/j.foodchem.2021.129783.
[18] Nie C N, Zhong X X, He L, et al.Comparison of different aroma-active compounds of Sichuan Dark brick tea (Camellia sinensis) and Sichuan Fuzhuan brick tea using gas chromatography-mass spectrometry (GC-MS) and aroma descriptive profile tests[J]. European Food Research & Technology, 2019, .245(9): 1963-1979.
[19] Wang J T, Shi J, Zhu Y, et al.Insights into crucial odourants dominating the characteristic flavour of citrus-white teas prepared from citrus reticulata Blanco ‘Chachiensis’ and Camellia sinensis ‘Fudingdabai’[J]. Food Chemistry, 2022, 377: 132048. doi: 10.1016/j.foodchem.2022.132048.
[20] 刘琳燕. 贮藏白茶的品质特性与清除自由基能力的研究[D]. 福州: 福建农林大学, 2015.
Liu L Y.Studies on the quality and the free radical scavenging activities of storage of white tea[D]. Fuzhou: Fujian Agriculture and Forestry University, 2015.
[21] Li Q, Li Y D, Luo Y, et al.Characterization of the key aroma compounds and microorganisms during the manufacturing process of Fu brick tea[J]. LWT, 2020, 127: 109355. doi: 10.1016/j.lwt.2020.109355.
[22] Yun J, Cui C J, Zhang S H, et al.Use of headspace GC/MS combined with chemometric analysis to identify the geographic origins of black tea[J]. Food Chemistry, 2021, 360(11):130033. doi: 10.1016/j.foodchem.2021.130033.
[23] Wu H T, Chen Y Y, Feng W Z, et al.Effects of three different withering treatments on the aroma of white tea[J]. Foods, 2022, 11(16): 2502. doi: 10.3390/foods11162502.
[24] Liao X L, Yan J N, Wang B, et al.Identification of key odorants responsible for cooked corn-like aroma of green teas made by tea cultivar 'Zhonghuang 1'[J]. Food Research International, 2020, 136: 109355. doi: 10.1016/j.foodres.2020.109355.
[25] Ni H, Jiang Q, Lin Q, et al.Enzymatic hydrolysis and auto-isomerization during β-glucosidase treatment improve the aroma of instant white tea infusion[J]. Food Chemistry, 2021, 342(2): 128565. doi: 10.1016/j.foodchem.2020.128565.
[26] Narbona E, Garcia E G, Araújo L V, et al.Volatile composition of functional ‘a la Piedra’ turrón with propolis[J]. International Journal of Food Science & Technology, 2010, 45(3): 569-577.
[27] 张应根, 陈林, 陈泉宾, 等. 基于特征香气的白茶香气品质评价模型构建初探[J]. 茶叶学报, 2017, 58(4): 149-156.
Zhang Y G, Chen L, Chen Q B, et al.Aromatics-based model for quality evaluation of white teas[J]. Acta Tea Sinica, 2017, 58(4): 149-156.
[28] 周巨根, 刘祖生, 洪健, 等. 茶树芽叶茸毛的研究——Ⅰ.形态特征及品种间差异[J]. 茶叶, 1988, (3): 13-16, 57.
Zhou J G, Liu Z S, Hong J, et al.A study on the hairy bud leaves of tea trees: Ⅰ. morphological characteristics and differences among cultivars[J]. Journal of Tea, 1988(3): 13-16, 57.
[29] 刘琳燕, 周子维, 邓慧莉, 等. 不同年份白茶的香气成分[J]. 福建农林大学学报(自然科学版), 2015, 44(1): 27-33.
Liu L Y, Zhou Z W, Deng H L, et al.Analysis of the aromatic components in white tea produced in different years[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2015, 44(1): 27-33.
[30] Song H L, Liu J B.GC-O-MS technique and its applications in food flavor analysis[J]. Food Research International, 2018, 114: 187-198.
[31] Bi J C, Li Y, Yang Z, et al.Effect of different cooking times on the fat flavor compounds of pork belly[J]. Journal of Food Biochemistry, 2022, 46(8): e14184. doi: 10.1111/jfbc.14184.
[32] Zhu J C, Niu Y W, Xiao Z B.Characterization of the key aroma compounds in Laoshan green teas by application of odour activity value (OAV), gas chromatography-mass spectrometry-olfactometry (GC-MS-O) and comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-qMS)[J]. Food Chemistry, 2021, 339(1): 128136. doi: 10.1016/j.foodchem.2020.128136.
[33] Wang M Q, Ma W J, Shi J, et al.Characterization of the key aroma compounds in Longjing tea using stir bar sorptive extraction (SBSE) combined with gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), odor activity value (OAV), and aroma recombination[J]. Food Research International, 2020, 130: 108908. doi: 10.1016/j.foodres.2019.108908.
[34] Guo X Y, Ho C T, Schwab W, et al.Aroma profiles of green tea made with fresh tea leaves plucked in summer[J]. Food Chemistry, 2021, 363: 130328. doi: 10.1016/j.foodchem.2021.130328.
[35] Feng T, Yang M Y, Ma B W, et al.Volatile profiles of two genotype Agaricus bisporus species at different growth stages[J]. Food Research International, 2021, 140: 109761. doi: 10.1016/j.foodres.2020.109761.
[36] Zhu Y, Lv H P, Shao C Y, et al.Identification of key odorants responsible for chestnut-like aroma quality of green teas[J]. Food Research International, 2018, 108: 74-82.
[37] Gao C X, Huang Y, Li J, et al.Relationship between the grade and the characteristic flavor of PCT (Panyong congou black tea)[J]. Foods, 2022, 11(18): 2815. doi: 10.3390/foods11182815.
[38] Lourencetti N M S, Wolf I R, Lacerda M P F, et al. Transcriptional profile of a bioethanol production contaminant Candida tropicalis[J]. AMB Express, 2018, 8(1): 166. doi: 10.1186/s13568-018-0693-1.
[39] Hara T, Kubota E.Changes in aroma components of green tea after storage[J]. Nippon Någeikagaku Kaishi, 1982, 56(8): 625-630.
[40] Polat A, Şat İ G, Ilgaz Ş, et al.Comparison of black tea volatiles depending on the grades and different drying temperatures[J]. Journal of Food Processing & Preservation, 2018, 42(7): e13653. doi: 10.1111/jfpp.13653.
[41] Wang J, Wu B, Zhang N, et al.Dehydration-induced carotenoid cleavage dioxygenase 1 reveals a novel route for β-ionone formation during tea (Camellia sinensis) withering[J]. Journal of Agricultural and Food Chemistry, 2020, 68(39): 10815-10821.
[42] Yue C, Cao H L, Zhang S R, et al.Aroma characteristics of Wuyi rock tea prepared from 16 different tea plant varieties[J]. Food Chemistry: X, 2023, 17: 100586. doi: 10.1016/j.fochx.2023.100586.
[43] Qi D D, Miao A Q, Cao J X, et al.Study on the effects of rapid aging technology on the aroma quality of white tea using GC-MS combined with chemometrics: in comparison with natural aged and fresh white tea[J]. Food Chemistry, 2018, 265: 189-199.
[44] Lin Q, Ni H, Wu L, et al.Analysis of aroma-active volatiles in an SDE extract of white tea[J]. Food Science & Nutrition, 2021, 9(2): 605-615.
[45] Wang C, Zhang C X, Kong Y W, et al.A comparative study of volatile components in Dianhong teas from fresh leaves of four tea cultivars by using chromatography-mass spectrometry, multivariate data analysis, and descriptive sensory analysis[J]. Food Research International, 2017, 100: 267-275.
[46] Wang X Q, Zeng L T, Liao Y Y, et al.An alternative pathway for the formation of aromatic aroma compounds derived from l-phenylalanine via phenylpyruvic acid in tea (Camellia sinensis (L.) O. Kuntze) leaves[J]. Food Chemistry, 2019, 270: 17-24.
[47] Luo Z M, Ling T J, Li L X, et al.A new norisoprenoid and other compounds from Fuzhuan brick tea[J]. Molecules, 2012, 17(3): 3539-3546.