湖北巴东县野生茶树种质资源遗传多样性及种群结构亲缘关系分析

doi:10.13305/j.cnki.jts.2024.02.002

茶叶科学 ›› 2024, Vol. 44 ›› Issue (2): 193-206.doi: 10.13305/j.cnki.jts.2024.02.002

湖北巴东县野生茶树种质资源遗传多样性及种群结构亲缘关系分析

崔清梅¹, 梁金波¹, 马会杰¹, 胡双玲¹, 陈清华¹, 吴立赟², 何梦迪², 王留彬², 谭立才³, 张强^1,*, 王丽鸳^2,*

1.恩施土家族苗族自治州农业科学院,湖北恩施 445000;
2.中国农业科学院茶叶研究所,浙江杭州 310008;
3.湖北正山堂巴东红茶业有限责任公司,湖北巴东 444300

收稿日期:2023-09-23 修回日期:2023-12-11 出版日期:2024-04-15 发布日期:2024-04-30
通讯作者: * 289367900@qq.com;wangly@tricaas.com
作者简介:崔清梅,女,正高级农艺师,主要从事茶树育种和栽培相关研究。
基金资助:
湖北省自然科学基金项目（2023AFD065）、国家茶叶产业技术体系（CARS-19）、湖北省重点研发计划项目（2023BBB179）

Genetic Diversity and Population Structure Relationship Analysis of Wild Tea Germplasm Resources in Badong County, Hubei Province

CUI Qingmei¹, LIANG Jinbo¹, MA Huijie¹, HU Shuangling¹, CHEN Qinghua¹, WU Liyun², HE Mengdi², WANG Liubin², TAN Licai³, ZHANG Qiang^1,*, WANG Liyuan^2,*

1. Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, China;
2. Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Hubei Zhengshantang Badong Black Tea Co., Ltd., Hubei Province, Badong 444300, China

Received:2023-09-23 Revised:2023-12-11 Online:2024-04-15 Published:2024-04-30

摘要/Abstract

摘要： 野生茶树种质资源具有较高的遗传多样性,是开展茶树品种选育的优质基因库。收集了来自湖北省巴东县的26份野生茶树资源,利用简单重复序列（Simple sequence repeat,SSR）分子标记并结合对照栽培型茶树品种对野生茶树资源的遗传多样性及种群结构等进行分析。结果如下：（1）16对引物在供试材料中共扩增得到82个等位位点,每对引物扩增所得的等位基因范围为3~8个,平均检测出等位位点5.12个,有效位点数量3.65个,香农多样性指数平均值1.378。（2）从16对引物中筛选出6个核心引物位点,可对26份野生茶树进行有效检测并鉴别。（3）UPGMA进化树将48份材料分为7个类别,野生茶树与栽培型茶树能通过SSR标记进行有效划分;进一步种群遗传结构分析发现26份野生茶树可分为2个亚群。（4）依据生化成分含量,筛选得出2份高EGCG含量的茶树种质资源及2份适制红茶的茶树种质资源。研究结果显示,巴东野生茶树多样性丰富,种群内部遗传变异高,为进一步保护、开发和利用巴东野生茶树种质资源奠定了基础。

关键词: 野生茶树, 多样性, 简单重复序列, 亲缘关系

Abstract: Wild tea germplasm resources have high genetic diversity and are also a high-quality source for the breeding and utilization of local tea cultivars. In this study, 26 resources of wild tea plants from Badong County, Hubei Province, were collected and the genetic diversity and population structure of wild tea plants were analyzed using SSR molecular markers with the normal tea cultivars as the control. The results are as follows: (1) 16 pairs of primers detected an average of 5.12 alleles and 3.65 effective loci in the test materials. A total of 82 alleles were amplified, with each pair of primers amplifying a range of 3-8 labeled alleles. The average Shannon diversity index was 1.378. (2) Six core primer sites were selected from 16 pairs of primers, which can effectively detect and identify 26 materials in this study. (3) UPGMA evolutionary map of individual samples could divide all 48 materials into 7 categories. Wild tea plants and cultivated tea plants could be effectively divided through SSR detection. Population genetic structure analysis suggests that 26 wild tea samples could be divided into 2 subgroups. (4) Based on biochemical components, two samples with high EGCG content and two tea germplasms suitable for making black tea were selected. Results of this research show that diversity level of wild tea resources in Badong was high, with high genetic variation within the population. This study laid a foundation for further protection, development and utilization of wild tea germplasm resources in Badong.

Key words: wild tea plant, diversity, SSR, genetic relationship

中图分类号:

S571.1
S324

崔清梅, 梁金波, 马会杰, 胡双玲, 陈清华, 吴立赟, 何梦迪, 王留彬, 谭立才, 张强, 王丽鸳. 湖北巴东县野生茶树种质资源遗传多样性及种群结构亲缘关系分析[J]. 茶叶科学, 2024, 44(2): 193-206. doi: 10.13305/j.cnki.jts.2024.02.002.

CUI Qingmei, LIANG Jinbo, MA Huijie, HU Shuangling, CHEN Qinghua, WU Liyun, HE Mengdi, WANG Liubin, TAN Licai, ZHANG Qiang, WANG Liyuan. Genetic Diversity and Population Structure Relationship Analysis of Wild Tea Germplasm Resources in Badong County, Hubei Province[J]. Journal of Tea Science, 2024, 44(2): 193-206. doi: 10.13305/j.cnki.jts.2024.02.002.

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湖北巴东县野生茶树种质资源遗传多样性及种群结构亲缘关系分析

Genetic Diversity and Population Structure Relationship Analysis of Wild Tea Germplasm Resources in Badong County, Hubei Province

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