福建野生茶树资源嘌呤生物碱构成评价及特异资源筛选

茶叶科学 ›› 2022, Vol. 42 ›› Issue (1): 18-28.

福建野生茶树资源嘌呤生物碱构成评价及特异资源筛选

陈潇敏¹, 赵峰^2,*, 王淑燕¹, 邵淑贤¹, 吴文晞³, 林钦⁴, 王鹏杰¹, 叶乃兴^1,*

1.福建农林大学园艺学院/茶学福建省高校重点实验室,福建福州 350002;
2.福建中医药大学药学院,福建福州 350122;
3.福建恒正检测技术有限公司,福建福州 350100;
4.福建省产品质量检验研究院,福建福州 350014

收稿日期:2021-06-02 修回日期:2021-10-09 出版日期:2022-02-15 发布日期:2022-02-18
通讯作者: *zhaofeng0591@fjtcm.edu.cn;ynxtea@126.com
作者简介:陈潇敏,女,硕士研究生,主要从事茶树栽培育种方面的研究。
基金资助:
茶学福建省高校重点实验室开放课题（KLTS2018002）、福建中医药大学引进人才科研启动项目（2801/701190097）、福建农林大学优秀硕士学位论文资助基金（1122YS01003）

Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea

CHEN Xiaomin¹, ZHAO Feng^2,*, WANG Shuyan¹, SHAO Shuxian¹, WU Wenxi³, LIN Qin⁴, WANG Pengjie¹, YE Naixing^1,*

1. College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian province, Fuzhou 350002, China;
2. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;
3. Hengzheng Testing Technology Company, Fuzhou 350100, China;
4. Fujian Institute of Product Quality Inspection, Fuzhou 350014, China

Received:2021-06-02 Revised:2021-10-09 Online:2022-02-15 Published:2022-02-18

摘要/Abstract

摘要： 以43份福建野生茶树种质资源为供试材料,通过超高效液相色谱串联质谱（UHPLC-MS/MS）测定其嘌呤生物碱构成,并经描述统计分析及聚类分析后,系统地分析了其特征属性。结果显示,参试资源样的嘌呤生物碱构成差异明显,大致可划归4个具体类群：类群Ⅰ嘌呤生物碱总量及构成接近于常见茶树品种;类群Ⅱ嘌呤生物碱较高,且主要由咖啡碱构成,仅含少量苦茶碱和可可碱;类群Ⅲ嘌呤生物碱较高,且苦茶碱与咖啡碱含量相当,仅含少量可可碱;类群Ⅳ嘌呤生物碱高,且构成以苦茶碱为主,咖啡碱和可可碱都较低。经综合评价后,从中筛得嘌呤生物碱构成较特异资源14份,包括高嘌呤生物碱（>60 mg·g^-1）5份,高咖啡碱（>50 mg·g^-1）7份,高苦茶碱（>30 mg·g^-1）7份。本研究可为茶树的种质资源保护、创新、品种选育及生产利用提供更多种质资源信息和科学依据。

关键词: 茶树, 种质资源, 嘌呤生物碱, 苦茶碱, 液质联用

Abstract: A total of forty-three wild tea resources in Fujian Province were applied in the research and the contents of purine alkaloids were determined by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The descriptive statistical analysis and cluster analysis were carried out to systematically analyze and explore the composition characteristics of purine alkaloids in Fujian wild tea resources. The results show that large variations in the purine alkaloids were identified in tea resources, which could be divided into four specific groups: group Ⅰ with similar contents of purine alkaloids as those in normal tea cultivars, group Ⅱ with higher purine alkaloids especially high caffeine content and low theophylline and theobromine, group Ⅲ with higher purine alkaloids especially high bitter theophylline and theophylline contents, group Ⅳ with high purine alkaloids especially bitter theophylline. After comprehensive evaluation, 14 tea resources with specific purine alkaloid composition were identified, including five germplasm resources with high purine alkaloids (>60 mg·g^-1), seven germplasm resources with high caffeine (>50 mg·g^-1) and seven germplasm resources with high bitter theophylline (>30 mg·g^-1). Through this study, it provided more information and a scientific basis for the protection, innovation, breeding, production and utilization of tea germplasm resources.

Key words: tea plant, germplasm resources, purine alkaloid, theacrine, liquid chromatography-mass spectrometry

中图分类号:

陈潇敏, 赵峰, 王淑燕, 邵淑贤, 吴文晞, 林钦, 王鹏杰, 叶乃兴. 福建野生茶树资源嘌呤生物碱构成评价及特异资源筛选[J]. 茶叶科学, 2022, 42(1): 18-28.

CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea[J]. Journal of Tea Science, 2022, 42(1): 18-28.

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