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茶叶科学 ›› 2012, Vol. 32 ›› Issue (1): 37-43.doi: 10.13305/j.cnki.jts.2012.01.005

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利用固定化纤维素酶酶解夏季绿茶工艺的研究

汪珈慧, 李燕*, 姚紫涵, 王家健   

  1. 上海海洋大学食品学院,上海 201306
  • 收稿日期:2011-04-20 修回日期:2011-06-21 出版日期:2012-02-15 发布日期:2019-09-04
  • 通讯作者: liyan@shou.edu.cn
  • 作者简介:汪珈慧(1987— ),女,上海人,在读研究生,主要从事活性物质提取方面的研究。
  • 基金资助:
    骆肇荛科创基金项目第五期

Study on the Influence of Immobilized Cellulase on the Summer Tea Extract

WANG Jia-hui, LI Yan*, YAO Zi-han, WANG Jia-jian   

  1. College of food and science technology,Shanghai Ocean University, Shanghai 201306, China
  • Received:2011-04-20 Revised:2011-06-21 Online:2012-02-15 Published:2019-09-04

摘要: 以壳聚糖作为载体,戊二醛为交联剂制备固定化纤维素酶,对夏季绿茶进行酶解。通过正交实验得到优化工艺:在1.0g茶叶中,以茶水比1︰20,加入1.0g固定化纤维素酶,55℃下恒温浸提50min。与传统高温水提法所得茶汤相比,以此工艺条件浸提所得茶汤中茶多酚、氨基酸、咖啡碱含量有所提高,酚氨比有所下降,连续提取7批次茶叶,固定化纤维素酶的相对酶活力仍保持80%以上。

关键词: 壳聚糖, 纤维素酶, 固定化酶, 夏季茶叶

Abstract: Immobilization of cellulase on carrier of chitosan beeds crosslinked by glutaraldehyde were studied, and applied the immobilized cellulase to the hydrolysis of the summer green tea. Through the orthogonal test, the optimal extract technology has been investigated: adding 1.0g immobilized cellulose enzyme in 1.0g green tea, the ratio of tea and water is 1∶20,and extracted 50min under 55℃. Compared to the traditional tea extraction method by hot water, the contents of tea polyphenols, amino acid and caffeine increased by using this technology, and the ratio of polyphenol to ammo acid was decreased. The immobilized cellulase still maintain a high activity, the relative activity of enzyme was more than 80%, after 7 times extraction.

Key words: chitosan, cellulose, immobilized enzyme, summer tea

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