基于茶树SNP的dCAPS标记体系研究

doi:10.13305/j.cnki.jts.2012.06.013

茶叶科学 ›› 2012, Vol. 32 ›› Issue (6): 517-522.doi: 10.13305/j.cnki.jts.2012.06.013

基于茶树SNP的dCAPS标记体系研究

张成才¹, 王丽鸳¹, 韦康¹, 成浩^1*, 包云秀², 刘本英², 汪云刚²

1. 中国农业科学院茶叶研究所,国家茶树改良中心,浙江杭州 310008;
2. 云南省农业科学院茶叶研究所,云南勐海 666201

收稿日期:2012-03-20 修回日期:2012-04-09 出版日期:2012-12-15 发布日期:2019-09-05
通讯作者: chenghao@mail.tricaas.com
作者简介:张成才（1986— ）,男,山东青岛人,硕士研究生,主要从事茶树种质资源与育种研究。
基金资助:
现代农业产业技术体系建设专项资金（No. nycytx-23）、中央级公益性科研院所基本科研业务费专项（2012Z L049）、浙江省自然科学基金（X307603）、浙江省茶产业技术创新战略联盟专项资金

Study of SNP and Relative dCAPS Markers in Tea Plant (Camellia sinensis)

ZHANG Cheng-cai¹, WANG Li-yuan¹, WEI Kang¹, CHENG Hao^1*, BAO Yun-xiu², LIU Ben-ying², WANG Yun-gang²

1. Tea research institute, Chinese Academy of Agriculture Science, National Center for Tea Improvement, Hangzhou 310008, China;
2. Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai 666201, China

Received:2012-03-20 Revised:2012-04-09 Online:2012-12-15 Published:2019-09-05

摘要/Abstract

摘要： 从茶树EST序列中搜寻出候选SNPs位点,在候选SNPs两侧设计测序引物进行PCR扩增,并对扩增产物双向测序验证;然后从每个扩增子中选择一个确证的SNPs,设计dCAPS引物,并进行扩增和酶切验证,将SNPs转化为dCAPS标记;最后,在长叶白毫×福鼎大白的F1群体中检测了标记的分离情况。结果发现,20对测序引物中有11对扩增成功,共确证了17个SNPs,占检测总数的54.8%;在设计的11对dCAPS引物中,有8对在长叶白毫、福鼎大白和龙井43等8个品种中表现出多态性,成功转化为dCAPS标记,转化成功率72.7%;8个dCAPS标记中的DCC229和DCC371在长叶白毫和福鼎大白之间表现有多态性,经检测,它们在这2个品种的F1群体中均符合孟德尔1︰1分离。

关键词: 茶树, EST, SNP, dCAPS

Abstract: SNPs were mined from tea-derived EST sequences. Primers were designed to evaluate the predicted SNPs in genomic DNA from tea plant. The PCR products were sequenced from both directions to confirm the valid SNPs. Meanwhile, the dCAPS primers were designed based on the valid SNPs and the genomic DNA was used for amplification. The amplicons were digested by restrict enzymes to convert SNPs into dCAPS markers. Furthermore, we also detected the separation of the new markers in a F1 population. The results showed that seventeen SNPs (54.8%) were confirmed in 11 amplycons, eight SNPs were successfully converted to dCAPS markers (72.7%), two of them which have polymorphisms in parents exhibited a separate ratio of 1︰1 in the F1 population. The new markers would be useful in tea genetic research. It is also believed that dCAPS technology will promote the use of SNP technology in genetics and breeding research in tea plant.

Key words: tea, EST, SNP, dCAPS

中图分类号:

S571.1
Q52

张成才, 王丽鸳, 韦康, 成浩, 包云秀, 刘本英, 汪云刚. 基于茶树SNP的dCAPS标记体系研究[J]. 茶叶科学, 2012, 32(6): 517-522. doi: 10.13305/j.cnki.jts.2012.06.013.

ZHANG Cheng-cai, WANG Li-yuan, WEI Kang, CHENG Hao, BAO Yun-xiu, LIU Ben-ying, WANG Yun-gang. Study of SNP and Relative dCAPS Markers in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2012, 32(6): 517-522. doi: 10.13305/j.cnki.jts.2012.06.013.

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基于茶树SNP的dCAPS标记体系研究

Study of SNP and Relative dCAPS Markers in Tea Plant (Camellia sinensis)

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