欢迎访问《茶叶科学》,今天是

茶叶科学 ›› 2006, Vol. 26 ›› Issue (4): 249-252.doi: 10.13305/j.cnki.jts.2006.04.003

• • 上一篇    下一篇

广东茶树种质资源AFLP分析

赵超艺1, 3, 周李华1, 4, *, 罗军武1, 黄建安1, 2, 谭和平4   

  1. 1. 湖南农业大学园林园艺学院茶学系,湖南 长沙 410128;
    2. 教育部茶学重点实验室,湖南 长沙 410128;
    3. 广东省农业科学院茶叶研究所,广东 英德 5103042;
    4. 中国测试技术研究院,四川 成都 610021
  • 收稿日期:2006-04-17 修回日期:2006-07-17 出版日期:2006-12-25 发布日期:2019-09-11
  • 通讯作者: * 周李华, E-mail:zhouxu1103@163.com
  • 作者简介:赵超艺(1962- ),男,副研究员,主要研究方向茶树资源利用与研究
  • 基金资助:
    广东省农科院资助项目(SR0273)

AFLP Analysis of Genetic Diversity of Tea plant Germplasms in Guangdong Province

ZHAO Chao-yi, LUO Jun-wu1, HUANG Jian-an1, 2, TAN He-ping4   

  1. 1. Tea Science Department, Hunan Agricultural University, ChangSha 410128, China;
    2. Key Laboratory of Tea Science of Ministry of Education, ChangSha 410128, China;
    3. Tea Research Institute, GuangDong Academy of Agricultural Sciences, YingDe 513042, China;
    4. National measure of institute and testing technology, ChengDu 610021, China
  • Received:2006-04-17 Revised:2006-07-17 Online:2006-12-25 Published:2019-09-11

摘要: 本研究采用AFLP技术,选用多态性高分辨能力强的5对引物对25个广东茶树群体品种和5个对照品种进行选择性扩增,获得了较清晰的DNA指纹图谱。5对引物共扩增出365条带,平均每对引物扩增73条带,多态性条带338条,占总带数的92.6%。扩增片段大小在50~600βbp之间。采用DPS 2000软件进行聚类,30份供试材料以清凉山茶和清远笔架茶遗传距离最近(0.12752),桂北大叶种和凤凰水仙之间的遗传距离最远(0.5),本实验结果表明广东地方茶树群体品种遗传多样性比较丰富。

关键词: 茶树, AFLP, 遗传分析

Abstract: Based on AFLP method, AFLP figure were obtained in Guangdong tea plants. In this study, totally 365 bands were amplified by 5 pairs of primes and 73 bands were amplified by each primer pair on average, a total of 338 (92.6%) polymorphic AFLP markers were detected. The results indicated that the genetic distance (GD) based on AFLP data by using DPS 2000 was significant. The GD(0.5) is highest between Guibeidaye and Fenghuangshuixian, but the GD(0.12752) between Qingliangshan tea and Qingyuanbijia tea is low. This indicated that the Guangdong tea plant has a high genetic diversity.

Key words: tea plant[Camellia sinensis(L.)O.Kuntze], AFLP, genetic diversity

中图分类号: