关键词: EpNPV, PCR, 基因, 分子鉴定

Abstract: According to nucleotide sequences of the two key genes, A13-1 lef-8 gene and A13-1 polyhedrin gene of EpNPV, two pairs of special primers were designed. Two typical DNA fragments with the size of 538 bp and 281 bp were amplified by PCR method. The two PCR products were cloned into T vector and sequenced, Blasted with the DNA sequence of EpNPV, both of them were matched absolutely. That meant that these two DNA fragments can be used as molecule markers to identify the Euproctis pseudoconspersa Strand infected with EpNPV. The present investigation constructed a molecular biological method to detect EpNPV DNA, to evaluate the biological control effect of EpNPV and to study the infecting route of EpNPV into E. pseudoconspera.

Key words: EpNPV, PCR, gene, molecule identification