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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (1): 53-58.doi: 10.13305/j.cnki.jts.2012.01.012

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mRNA Differential Expressionof Camellia sinensis under Drought Conditions

CHEN Sheng-xiang, QI Gui-nian*, XIA Jian-bing, ZOU Yao, SHAN Hong-li   

  1. Department of tea Science, College of Horticulture, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2011-09-13 Revised:2011-11-01 Online:2012-02-15 Published:2019-09-04

Abstract: 20% PEG-6000 was used for simulating drought treatment to Camellia sinensis cv. Fuding-dabaicha clone. mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) was used to study the mRNA differential expression between drought stress and irrigated contro1. One down regulated gene was found under drought stress. Sequence analysis and homology alignment showed that N3-5 showed 75% homogenicity with the Camellia sinensis clone Sajin tea leaf mutant color tag S31.B15 genomic sequence (Accession:DQ443473.1). It was suggested that the fragments N3-5 was related to drought tolerance mechanism of tea plant according to the functions of their homologous sequences.

Key words: Camellia sinensis, drought stress, mRNA differential expression

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