mRNA Differential Expressionof Camellia sinensis under Drought Conditions

CHEN Sheng-xiang; QI Gui-nian; XIA Jian-bing; ZOU Yao; SHAN Hong-li

doi:10.13305/j.cnki.jts.2012.01.012

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (1) : 53-58. DOI: 10.13305/j.cnki.jts.2012.01.012

mRNA Differential Expressionof Camellia sinensis under Drought Conditions

CHEN Sheng-xiang, QI Gui-nian^*, XIA Jian-bing, ZOU Yao, SHAN Hong-li

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Abstract

20% PEG-6000 was used for simulating drought treatment to Camellia sinensis cv. Fuding-dabaicha clone. mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) was used to study the mRNA differential expression between drought stress and irrigated contro1. One down regulated gene was found under drought stress. Sequence analysis and homology alignment showed that N_3-5 showed 75% homogenicity with the Camellia sinensis clone Sajin tea leaf mutant color tag S31.B15 genomic sequence (Accession：DQ443473.1). It was suggested that the fragments N_3-5 was related to drought tolerance mechanism of tea plant according to the functions of their homologous sequences.

Key words

Camellia sinensis / drought stress / mRNA differential expression

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CHEN Sheng-xiang, QI Gui-nian, XIA Jian-bing, ZOU Yao, SHAN Hong-li. mRNA Differential Expressionof Camellia sinensis under Drought Conditions[J]. Journal of Tea Science. 2012, 32(1): 53-58 https://doi.org/10.13305/j.cnki.jts.2012.01.012

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