Cloning and Expression Analysis of Betaine Aldehyde Dehydragenase Gene (CsBADH1) from Tea Plant [Camellia sinensis (L.) O. Kuntze]

CAO Hong-li; HAO Xin-yuan; YUE Chuan; MA Chun-lei; WANG Xin-chao; YANG Ya-jun

doi:10.13305/j.cnki.jts.2013.02.009

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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (2) : 99-108. DOI: 10.13305/j.cnki.jts.2013.02.009

Cloning and Expression Analysis of Betaine Aldehyde Dehydragenase Gene (CsBADH1) from Tea Plant [Camellia sinensis (L.) O. Kuntze]

CAO Hong-li^1,2, HAO Xin-yuan^1,3, YUE Chuan^1,2, MA Chun-lei¹, WANG Xin-chao^1,*, YANG Ya-jun^1,*

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Abstract

Betaine is nontoxic osmotic solute which can accumulate as cryoprotectant molecule in response to environment stress in plants. Betaine aldehyde dehydragenase (BADH) is one of the key enzymes involving in the betaine biosynthesis pathway. A full length cDNA sequence of BADH gene was cloned by SMART RACE-PCR from tea plant, which was named as CsBADH1(GenBank Accession No. JX050145). The bioinformatic characterization indicated that the full length cDNA of CsBADH1 was 1972 bp,which contained 1β518βbp complete CDS and encoded 505 amino acid residues with a putative molecular mass of 54.8βkD and an isoionic point of 5.652. It belongs to a hydrophobic protein. The deduced amino acid sequence contained the conserved decapeptide (VTLELGGKSP) and cysteine residue(C) in aldehyde dehydrogenase (ALDHs). Phylogenetic analysis showed that CsBADH1 was most closely to Panax ginseng with 87% amino acids similarity, the most of other plants were also above 80%. Quantitative real-time PCR analysis further showed that the expression levels of CsBADH1 in acclimated samples were higher than non-acclimated samples (CK). It indicates that CsBADH1 may mediate the regulation of cold acclimation in tea plant.

Key words

betaine aldehyde dehydrogenase (BADH) / cold acclimation / expression analysis / gene clonging / tea plant (Camellia sinensis)

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CAO Hong-li, HAO Xin-yuan, YUE Chuan, MA Chun-lei, WANG Xin-chao, YANG Ya-jun. Cloning and Expression Analysis of Betaine Aldehyde Dehydragenase Gene (CsBADH1) from Tea Plant [Camellia sinensis (L.) O. Kuntze][J]. Journal of Tea Science. 2013, 33(2): 99-108 https://doi.org/10.13305/j.cnki.jts.2013.02.009

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