西藏墨脱引起茶树叶枯病的两种镰刀菌新病原鉴定和生物学特性

doi:10.13305/j.cnki.jts.2026.02.007

Abstract

Abstract: In June and July 2024, two Fusarium spp. strains were isolated from blighted leaves of tea plants (Camellia sinensis) in Motuo County, Linzhi City, Xizang, China. Species identification was conducted using morphological characteristics, multi-locus phylogenetic analysis, and Koch's postulates. The results identified the strains as Fusarium dracaenophilum and Fusarium asiaticum. Pathogenicity tests confirm that both species induce tea leaf blight. In varietal pathogenicity assays, ‘Zhonghuang 1’ exhibited significantly stronger resistance to both pathogens than ‘Fuding Dabaicha’. The results of the biological characterization reveal that F. asiaticum exhibits optimal growth conditions at temperatures ranging from 20 ℃ to 25 ℃ and at pH levels between 7 and 9. The presence of sucrose, maltose, lactose, and yeast extract has been demonstrated to stimulate F. asiaticum growth. F. dracaenophilum exhibits optimal growth at 25 ℃ and pH 7, with lactose and yeast extract being the most favorable nutrients. These findings enhanced understanding of Fusarium pathogen diversity and provided a theoretical basis for managing foliar diseases in tea plantations in Xizang.

Key words: tea leaf blight, Fusarium spp., morphological identification, multilocus phylogenetic analysis

CLC Number:

JIN Huai, CUI Ting, WANG Nan, WEI Liping, ZHANG Rong, GONG Wenfeng. Identification and Biological Characterization of Two Novel Fusarium Pathogen Causing Tea Leaf Blight in Motuo Conty, Xizang[J]. Journal of Tea Science, 2026, 46(2): 318-330.

References

[1] Mukhopadhyay M, Mondal T K, Chand P K.Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze): a review[J]. Plant Cell Reports, 2015, 35(2): 255-287.
[2] Jeyaraj A, Chandran V, Gajjeraman P.Differential expression of microRNAs in dormant bud of tea [Camellia sinensis (L.) O. Kuntze][J]. Plant Cell Reports, 2014, 33(7): 1053-1069.
[3] Tang G Y, Meng X, Gan R Y, et al.Health functions and related molecular mechanisms of tea components: an update review[J]. International Journal of Molecular Sciences, 2019, 20(24): 6196. doi: 10.3390/ijms20246196.
[4] 尤星宇, 冷杨, 霍春悦, 等. 2024年中国茶叶进出口贸易情况简析[J]. 中国茶叶, 2025, 47(3): 14-19.
You X Y, Leng Y, Huo C Y, et al.A brief analysis of tea import and export trade in China in 2024[J]. China Tea, 2025, 47(3): 14-19.
[5] Chen P F, Cai J R, Zheng P, et al.Quantitatively unravelling the impact of high altitude on oolong tea flavor from Camellia sinensis grown on the plateaus of Tibet[J]. Horticulturae, 2022, 8(6): 539. doi: 10.3390/horticulturae8060539.
[6] Hu G S, Wan M Z, Wei K, et al.Computer vision based method for severity estimation of tea leaf blight in natural scene images[J]. European Journal of Agronomy, 2023, 144: 126756. doi: 10.1016/j.eja.2023.126756.
[7] Shahriar S A, Nur-Shakirah A O, Mohd M H. Neopestalotiopsis clavispora and Pseudopestalotiopsis camelliae-sinensis causing grey blight disease of tea (Camellia sinensis) in Malaysia[J]. European Journal of Plant Pathology, 2021, 162(3): 709-724.
[8] Liu F, Ma Z Y, Hou L W, et al.Updating species diversity of Colletotrichum, with a phylogenomic overview[J]. Studies in Mycology, 2022, 101: 1. doi: 10.3114/sim.2022.101.01.
[9] Jeyaraj A, Elango T, Chen X, et al.Advances in understanding the mechanism of resistance to anthracnose and induced defence response in tea plants[J]. Molecular Plant Pathology, 2023, 24(10): 1330-1346.
[10] Guo M J, Zhao S Y, Gao Y, et al.A phylogenetic and taxonomic revision of Discula theae-sinensis, the causal agents of anthracnose on Camellia sinensis[J]. Journal of Fungi, 2024, 10(2): 141. doi: 10.3390/jof10020141.
[11] Crous P, Lombard L, Sandoval-Denis M, et al. Fusarium: more than a node or a foot-shaped basal cell[J]. Studies in Mycology, 2021, 98: 100106. doi: 10.1016/j.simyco.2021.100116.
[12] Sandoval-Denis M, Costa M M, Broders K, et al.An integrative re-evaluation of the Fusarium sambucinum species complex[J]. Studies in Mycology, 2025, 110: 1. doi: 10.3114/sim.2025.110.01.
[13] Xu W, Zhao F H, Deng X X, et al.First report of collar canker and dieback of Camellia sinensis caused by Fusarium solani species complex in Henan, China[J]. Plant Disease, 2022, 106(8): 2263. doi: 10.1094/pdis-07-21-1517-pdn.
[14] Zhang X Y, Yi J, Huo D, et al.First report of Fusarium proliferatum causing leaf spot on tea plants in Jiangxi Province, China[J]. Plant Disease, 2023, 107(7): 2220. doi: 10.1094/pdis-04-22-0896-pdn.
[15] 王文华. 墨脱县冬季气候与热带果树引种适应性[J]. 热带农业科学, 2020, 40(9): 39-44.
Wang W H.Adaptability of introduced tropical fruit trees to winter in Motuo County[J]. Chinese Journal of Tropical Agriculture, 2020, 40(9): 39-44.
[16] 朱罗娟, 江冰冰, 马玉青, 等. 西双版纳茶区茶轮斑病的致病病原分离及鉴定[J]. 茶叶科学, 2025, 45(6): 1047-1054.
Zhu L J, Jiang B B, Ma Y Q, et al.Isolation and identification of pathogens causing tea gray blight in Xishuangbanna tea area[J]. Journal of Tea Science, 2025, 45(6): 1047-1054.
[17] O'donnell K, Kistler H, Cigelnik E, et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies[J]. PNAS, 1998, 95(5): 2044-2049.
[18] Liu Y J, Whelen S, Hall B.Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse Ⅱ subunit[J]. Molecular Biology and Evolution, 1999, 16(12): 1799-1808.
[19] Reeb V, Lutzoni F, Roux C.Contribution of RPB2 to multilocus phylogenetic studies of the euascomycetes (Pezizomycotina, Fungi) with special emphasis on the lichen-forming Acarosporaceae and evolution of polyspory[J]. Molecular Phylogenetics and Evolution, 2004, 32(3): 1036-1060.
[20] O'donnell K, Sutton D A, Rinaldi M G, et al. Internet-accessible DNA sequence database for identifying Fusaria from human and animal infections[J]. Journal of Clinical Microbiology, 2010, 48(10): 3708-3718.
[21] O'donnell K, Nirenberg H, Aoki T, et al. A multigene phylogeny of the Gibberella fujikuroi species complex: detection of additional phylogenetically distinct species[J]. Mycoscience, 2000, 41: 61-78. doi: 10.1007/BF02464387.
[22] Han S L, Wang M M, Ma Z Y, et al. Fusarium diversity associated with diseased cereals in China, with an updated phylogenomic assessment of the genus[J]. Studies in Mycology, 2023, 104(1): 87-148.
[23] Zeng X Y, Tan T J, Tian F H, et al.OFPT: a one-stop software for fungal phylogeny[J]. Mycosphere, 2023, 14(1): 1730-1741.
[24] Katoh K, Standley D M.MAFFT multiple sequence alignment software version 7: improvements in performance and usability[J]. Molecular Biology and Evolution, 2013, 30(4): 772-780.
[25] Capella-Gutiérrez S, Silla-Martínez J M, Gabaldón T. TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses[J]. Bioinformatics, 2009, 25(15): 1972-1973.
[26] Kalyaanamoorthy S, Minh B Q, Wong T K F, et al. ModelFinder: fast model selection for accurate phylogenetic estimates[J]. Nature Methods, 2017, 14(6): 587-589.
[27] Nguyen L T, Schmidt H A, Von Haeseler A, et al.IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J]. Molecular Biology and Evolution, 2015, 32(1): 268-274.
[28] Bao Y M, Zhang Z, Zhao W M, et al.Database resources of the national genomics data center, China national center for bioinformation in 2025[J]. Nucleic Acids Research, 2024, 53(D1): D30-D44.
[29] Bu C F, Zheng X C, Zhao X T, et al. GenBase: a nucleotide sequence database [J]. Genomics, Proteomics & Bioinformatics, 2024, 22(3): qzae047. doi: 10.1093/gpbjnl/qzae047.
[30] 张婷, 王艳, 晋玲, 等. 当归根腐病主要病原菌生物学特性及室内毒力测定[J]. 微生物学杂志, 2024, 44(2): 71-78.
Zhang T, Wang Y, Jin L, et al.Biological characteristics and indoor toxicity test of main pathogens of Chinese Angelica (Angelica sinensis) root rot[J]. Journal of Microbiology, 2024, 44(2): 71-78.
[31] 靳启明, 闫成才, 王庆朋, 等. 6株核桃腐烂病菌的分子鉴定及生物学特性比较[J]. 山东农业科学, 2024, 56(12): 98-104.
Jin Q M, Yan C C, Wang Q P, et al.Molecular identification and comparison on biological characteristics of six walnut canker pathogens[J]. Shangdong Agricultural Science, 2024, 56(12): 98-104.
[32] 谢江涛, 覃再丽, 刘雪, 等. 毛木耳蛛网病致病菌生物学特性及杀菌剂敏感性分析[J]. 菌物学报, 2024, 43(8): 16-25.
Xie J T, Qin Z L, Liu X, et al.Biological characteristics and fungicide sensitivities of Hypomyces mycophilus causing cobweb disease of Auricularia cornea[J]. Mycosystema, 2024, 43(8): 16-25.
[33] Manawasinghe I S, Phillips A J L, Xu J P, et al. Defining a species in fungal plant pathology: beyond the species level[J]. Fungal Diversity, 2021, 109(1): 267-282.
[34] Manawasinghe I S, Jayawardena R S, Li H L, et al.Microfungi associated with Camellia sinensis: a case study of leaf and shoot necrosis on tea in Fujian, China[J]. Mycosphere, 2021, 12(1): 430-518.
[35] Fan Q, Su P P, Xiao J C, et al.Phylogenomic, morphological, and phylogenetic evidence reveals five new species and two new host records of Nectriaceae (Hypocreales) from China[J]. Biology, 2025, 14(7): 871. doi: 10.3390/biology14070871.
[36] 毛娟, 旺杰次仁, 罗英, 等. 林芝茶园土壤养分特征与肥力质量评价[J]. 江苏农业科学, 2023, 51(23): 211-218.
Mao J, Wangjie C R, Luo Y, et al.Soil nutritional status analysis and fertility assessment of tea plantations in Linzhi region[J]. Jiangsu Agricultural Sciences, 2023, 51(23): 211-218.
[37] 史田斌, 王多锋, 张露荷, 等. 陇南地区8个茶品种品质评价[J]. 甘肃农业大学学报, 2024, 59(6): 309-317.
Shi T B, Wang D F, Zhang L H, et al.Quality assessment of eight tea varieties in Longnan region[J]. Journal of Gansu Agricultural University, 2024, 59(6): 309-317.
[38] Sánchez-Hernández E, Andrés-Juan C, Buzón-Durán L, et al.Antifungal activity of methylxanthines against grapevine trunk diseases[J]. Agronomy, 2022, 12(4): 885. doi: 10.3390/agronomy12040885.
[39] Kurek J, Sierakowska A, Berdzik N, et al. Colchicine, caffeine, gramine, and their derivatives as potential herbicides, fungicides, and insecticides[J]. International Journal of Molecular Sciences, 2024, 25(18): 10081. doi: 10.3390/ijms251810081.
[40] Zhang Y Q, Wang F Y, Wang L J, et al.The response of growth and transcriptome profiles of tea grey blight disease pathogen Pestalotiopsis theae to the variation of exogenous L-theanine[J]. International Journal of Molecular Sciences, 2024, 25(6): 3493. doi: 10.3390/ijms25063493.

Identification and Biological Characterization of Two Novel Fusarium Pathogen Causing Tea Leaf Blight in Motuo Conty, Xizang

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