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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (2): 153-157.doi: 10.13305/j.cnki.jts.2005.02.013

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Analysis on Applicability of ISSR in Molecular Identification and Relationship Investigation of Tea Cultivars

YAO Ming-zhe2, HUANG Hai-tao2, YU Ji-zhong2, CHEN Liang1*   

  1. 1. Tea Research Institute, Chinese Academy of Agricultural, Sciences Hangzhou 310008, China;
    2. Tea Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
  • Online:2005-06-25 Published:2019-09-16

Abstract: Inter-simple sequence repeat (ISSR) is a recently developed PCR-based molecular technology. It has been widely used in genetic diversity, phylogenetic studies, gene tagging, genome mapping and evolutionary biology in a wide range of crop species due to its simple, quick, efficient, high reproducibility and low expense. In this study, the applicability of ISSR on molecular identification and relationship analysis of tea cultivars were investigated. A total of 99 bands were amplified by eight ISSR primers in six tea cultivars and the average polymorphism was 79.6%. The six tea cultivars could be clearly distinguished by ISSR fingerprinting amplified with primers TRI22 and TRI30, and also by the presence or absence of specific ISSR markers and their combination. Six cultivars were divided into two groups by cluster analysis. Maolu, Cuifeng, Qingfeng, Yingshuang and Jinfeng were in one group and Longjing 43 in the other. The relationship of six tea cultivars were validated by cluster analysis based on ISSR. The results indicate that ISSR is a useful tool for molecular identification and relationship analysis of tea cultivars.

Key words: Tea, Cultivars, ISSR, Molecular identification, Relationship

CLC Number: