Abstract: “Cold-making tea” is a new type of tea. To ensure the safety of cold-brewing tea, it is necessary to conduct the research on its property and safety. Results showed that the mice were ingested the tea sample by means of maximal tolerant dosage(40βml/kg·BW)method, The median acute oral lethal dose(LD₅₀)>40βml/kg·BW and it equals to LD₅₀>20βg/kg·BW of the dried tea sample. It can be classified as the class of nontoxic . Based on the above results, three-treatment levels（the maximum dosage 20βml/kg·BW）of the tea infusion were ingested into the mice stomach. It was showed that the result of mice micronucleual test of bone marrow cell was negitive. which means the tea sample was no teratogenicity on sperm and had no micronucleus effect on Kunming mice under the test levels. Results showed that the tea sample was nontoxic and safe.

Key words: acute toxicity, genetic toxicity, security, cold-brewing tea