基于SCoT标记的福建茶树品种（系）遗传多样性分析

doi:10.13305/j.cnki.jts.2018.01.005

Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (1): 43-57.doi: 10.13305/j.cnki.jts.2018.01.005

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Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers

LIN Weidong^1,2,4, CHEN Zhidan^2,3,4, SUN Weijiang^1,2,3,4,*, YANG Ruxing⁵

1. College of HorticuLture, Fujian AgricuLture and Forestry University, Fuzhou 350002, China;
2. Fujian-Taiwan Joint Centre for Ecological Control of Crop Pest, Fuzhou 350002, China;
3. Anxi College of Tea Science, Fujian AgricuLture and Forestry University, Fuzhou 350002, China;
4. Fujian Tea Industry Engineering Technology Research Center, Fuzhou 350002, China;
5. Tea Research Institute, Fujian Academy of AgricuLtural Sciences, Fu’an 355015, China

Received:2017-06-13 Revised:2017-08-24 Online:2018-02-15 Published:2019-08-28

Abstract

Abstract: SCoT-PCR amplification system is constructed to analyze the tea resources in Fujian. Totally 16 polymorphic primers were screened from 38 SCoT primers to construct fingerprints of 55 tea cultivars (lines). A total of 219 bands were amplified from 55 materials, with 216 polymorphic bands. The average bands amplified by each primer and polymorphic ratio were 13.8 and 93.15%. The Genetic similarity (GS) of 55 tea resources ranged from 0.49 to 0.85, with an average of 0.67. The SCoT marker analysis showed that the observed number of alleles was 1.93 in these two tea groups. The effective number of alleles, Nei gene diversity, Shannon’s information index, genetic differentiation and the gene flow were 1.54, 0.32, 0.48, 0.067 and 7.01, respectively. When the genetic similarity coefficient was set to 0.64, the tea resources could be divided into two major categories.

Key words: tea[Camellia sinensis(L.) O. Kuntze], SCoT, genetic diversity, genetic relationship

CLC Number:

S571.1
Q52

LIN Weidong, CHEN Zhidan, SUN Weijiang, YANG Ruxing. Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers[J]. Journal of Tea Science, 2018, 38(1): 43-57.

References 9

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Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers

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