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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (3): 367-375.doi: 10.13305/j.cnki.jts.2022.03.006

• Research Paper • Previous Articles     Next Articles

The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease

ZHANG Zhipeng, CHENG Qinghua, XIE Jingkang, WAN Yuhe, TONG Huarong, CHEN Yingjuan*   

  1. National Demonstration Center for Experimental Food Science and Technology Education, Department of Tea Science, College of Food Science, Southwest University, Chongqing 400715, China
  • Received:2021-12-20 Revised:2022-03-16 Online:2022-06-15 Published:2022-06-17

Abstract: To explore the application prospects of berberine on the prevention and control of tea diseases, the antifungal activity of berberine against five different Colletotrichum species (C. fioriniae, C. karstii, C. chongqinggense, C. camelliae and C. gloeosporioides) were studied and the differences in antifungal activities were compared. The results show that berberine has the best inhibitory effect on both C. camelliae and C. chongqinggense, reaching 100% at the concentration of 12 mg·mL-1, followed by C. gloeosporioides, C. karstii and C. fioriniae. The EC50 (Concentration for 50% of maximal effect) of the five Colletotrichum species were 2.828, 3.288, 4.164, 4.778 mg·mL-1 and 5.104 mg·mL-1, respectively. The conidial and hyphal morphology of five Colletotrichum species under the treatment of berberine were examined, showing that berberine had a significant impact on the mycelial and conidial growth of five Colletotrichum species. With the increase of berberine concentration, part of hyphae expanded irregularly and the conidia grew abnormally. Results of the biological activity of Colletotrichum species show that the cell structure was damaged, the permeability of cell membrane was increased and the cell oxidative stress response was enhanced. Results of this study firstly confirm the antifungal activity and application prospects of berberine against Colletotrichum species, which provided a new approach and theoretical basis for the prevention and control of tea disease caused by Colletotrichum.

Key words: berberine, Colletotrichum, antifungal activity, mechanism

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