用于测定黑茶中铅的荧光增强化学传感器

Abstract

Abstract: An optical chemical sensing membrane based on reversible fluorescence enhancement of Erythromycin A immobilized in a plasticized poly (vinyl chloride) (PVC) membrane for Lead (Ⅱ) detection was developed. The membrane of the sensor consists of 50 mg of PVC, 100 mg of Di (2-ethylhexyl) phthalate and 2.94 mg of Erythromycin A. The maximum response of the sensing membrane to Lead (Ⅱ) was obtained in Tris/HCl buffer solution ( pH 8.0). Under optimal conditions, the proposed sensor responded linearly to Lead (Ⅱ) in the range of 4.00-6.00 mmol/L and had a detection limit of 0.10 μmol·L^-1. The response time of the sensor was less than 50 s. In addition to the high reproducibility and reversibility of the fluorescence signal, the sensor also exhibited good selectivity. Except for Cr₂O₇^2-and MnO₄^-, it was not interfered by other common metal ions and anions. It was applied for the determination of Lead(Ⅱ) in a sample of FU Tea, which offered a satisfactory result.

Key words: optical chemical sensor, fluorescence enhancement, lead(Ⅱ), erythromycin A

CLC Number:

LONG Liping, MENG Wei, WANG Jiaoliang, XIE Dan, HE Guowen, XIAO Guqing. A Sensing PVC Membrane Based on Fluorescence Enhancement of Erythromycin A for Lead (Ⅱ) Detection in FU Tea[J]. Journal of Tea Science, 2017, 37(3): 273-279.

References 12

[1]	国家认证认可监督管理委员会. SN/T 2056—2008 进出口茶叶中铅、砷、镉、铜、铁含量的测定电感耦合等离子体原子发射光谱法[S]. 2008.
[2]	中华人民共和国卫生部. GB 5009. 12—2010 食品安全国家标准食品中铅含量的测定[S]. 2010.
[3]	杜保安, 刘澄, 曹雨虹, 等. CdTe量子点作荧光探针检测微量铅的方法研究[J]. 光谱学与光谱分析, 2013, 32(5): 1266-1269.
[4]	龙立平, 聂伟安, 钟桐生, 等. 基于卟啉衍生物荧光熄灭的Pb2+光化学传感器[J]. 应用化学, 2007, 24(7): 806-810.
[5]	赵永席, 齐林, 杨卫军, 等. 基于核酸切割酶与脱氧核酶的荧光循环放大系统检测铅(Ⅱ)[J]. 分析化学, 2012, 40(8): 1236-1240.
[6]	崔凤, 霍瑞娜, 张贵生, 等. 抗癌药物4'-O-(α-L-夹竹桃糖基)柔红霉素与小牛胸腺DNA的相互作用的荧光光谱[J]. 应用化学, 2012, 29(12): 238-244.
[7]	曾恚恚, 王柯敏, 俞汝勤, 等. 苦味酸荧光光化学传感器在药物分析中的应用[J]. 应用化学, 1994, 11(2): 48-52.
[8]	曾恚恚, 王柯敏, 秦臻, 等. 基于荧光熄灭的二氧化硫选择性光化学敏感膜的研究[J]. 高等学校化学学报, 1993, 14(2): 180-183.
[9]	段云青, 闵顺耕. 溴鼠灵与DNA作用机制的光谱研究[J]. 光谱学与光谱分析, 2009, 29(4): 999-1003.
[10]	YANG Ronghua, WANG Kemin, LONG Liping, et al.A selective optode membrane for histidine based on fluorescence enhancement of meso-meso-linked porphyrin dimer[J]. Anal Chem, 2002, 74(5): 1088-1096.
[11]	金鑫, 朱家文, 陈葵, 等. 缓冲盐溶液反萃分离红霉素A、C的研究[J]. 化学工程, 2013, 41(2): 29-32.
[12]	CHOI Martin M F, WU Xiaojun, LI Yourong. Optode membrane for determination of nicotine via generation of its bromoethane derivative[J]. Anal Chem, 1999, 71(7): 1342-1349.

[1]	XIAO Juanjuan, CHENG Ying, LIU Yan, LIU Qiaofang, JIANG Ating, HUANG Jian'an, WANG Kunbo, LIU Zhonghua, WANG Zhenhong, YU Lijun. Isolation and Identification of Aspergillus cristatus LJSC.2006 and Its Effect on Fu Tea’s Quality [J]. Journal of Tea Science, 2024, 44(4): 639-654.
[2]	GAO Chenxi, HUANG Yan, SUN Weijiang. Research Progress of Proanthocyanidins in Tea [J]. Journal of Tea Science, 2020, 40(4): 441-453.
[3]	MA Wanjun, MA Shicheng, LIU Chunmei, LONG Zhirong, TANG Baojun, LIN Zhi, LYU Haipeng. Research Progress on Chemical Composition and Biological Activity of Liupao Tea [J]. Journal of Tea Science, 2020, 40(3): 289-304.
[4]	ZENG Hongzhe, HUANG Xiangxiang, YU Lijun, ZHOU Yufei, XU Shuai, QU Furong. Effects of ‘Eurotium cristatum Loose Tea’ and ‘Eurotium cristatum Powder’ on the Expressions of JAK2/STAT3 Inflammation and Phosphorylated Proteins in Lung Tissue of Passive Smoking Mice [J]. Journal of Tea Science, 2020, 40(2): 165-172.
[5]	ZUO Gaolong, CHEN Meiyan, LIN Yong, LIN Haiyan, LIU Zhonghua, WU Wenliang. Protective Effect of Fu Brick Tea on Intestinal Mucosal Barrier and Liver Injury in NAFLD Rats [J]. Journal of Tea Science, 2019, 39(6): 631-640.
[6]	WANG Chunyan, XIAO Changyi, LI Shigang, TU Xuan, HE Jiangang. A Preliminary Research on Identification and Simulated Fermentation of the Mucor Fungi from Hubei Green Brick Tea [J]. Journal of Tea Science, 2018, 38(6): 634-642.
[7]	MA Yan, CHEN Lijiao, LYU Caiyou, ZHAO Ming. Review on the Studies about Safety Assessment of Pu-erh Tea [J]. Journal of Tea Science, 2018, 38(3): 221-226.
[8]	JIANG Yihe, XU Wei, ZHU Qi. Research Progress and Discussion on Fungal Contamination of Dark Tea [J]. Journal of Tea Science, 2018, 38(3): 227-236.
[9]	WU Genliang, HOU Aixiang, LI Ke, LI Zongjun. Effects of Polyphenols of Old Fu Brick Tea on the Elderly Intestinal Flora [J]. Journal of Tea Science, 2018, 38(3): 319-330.
[10]	WANG Ruru, XIAO Mengchao, LI Daxiang, LING Tiejun, XIE Zhongwen. Recent Advance on Quality Characteristics and Health Effects of Dark Tea [J]. Journal of Tea Science, 2018, 38(2): 113-124.
[11]	SHEN Wei, TENG Ruimin, LI Hui, LIU Zhiwei, WANG Yongxin, WANG Wenli, ZHUANG Jing. Cloning of a MADS-box Transcription Factor Gene from Camellia sinensis and its Response to Abiotic Stresses [J]. Journal of Tea Science, 2017, 37(6): 575-585.
[12]	YANG Chen, DAI Weidong, LYU Meiling, LI Pengliang, LIU Xu, TIAN Jun, WAN Yunlong, LI Ji, LIN Zhi. Study on the Chemical Constituents of Pu-erh Teas from Different Areas by UHPLC-Q-TOF/MS [J]. Journal of Tea Science, 2017, 37(6): 605-615.
[13]	XU Wei, JIANG Yihe, WU Dan, ZHAO Renliang, ZHU Qi. RNA Sequencing Analysis of Fungi Community Diversity in Mildew Raw Dark Tea [J]. Journal of Tea Science, 2017, 37(5): 483-492.
[14]	LIU Liping, TANG Yuwei, WANG Ruoxian, LI Zhibing, LIU Zhonghua, LIU Shuoqian. Production of Bioactive Indole Alkaloids through Fermention of Eurotium cristatum from Fuzhuan Tea [J]. Journal of Tea Science, 2017, 37(5): 503-512.
[15]	LI Yali, XING Qianqian, TU Qing, ZHOU Hongjie. Study on the Safety of Pu-erh Tea Contaminated by Exogenous Aspergillus flavus [J]. Journal of Tea Science, 2017, 37(5): 513-522.

A Sensing PVC Membrane Based on Fluorescence Enhancement of Erythromycin A for Lead (Ⅱ) Detection in FU Tea

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 12

Related Articles 15

Metrics

Comments

Recommended 0