茶树GAGP基因克隆及表达分析

doi:10.13305/j.cnki.jts.2015.01.012

Abstract

Abstract: By using SSH, the differences in gene expression of root from Fudingdabai tea plant infected by VA mycorrhiza were analyzed and the diversity sequences was obtained. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possibly contained 5 known genes. The GAGP (gap-pol) genic full-length sequence was obtained by using RACE. The length of GAGP gene was 3146bp (GenBank, Accession no., KJ946251), with 2β769βbp ORF (1^st-2β769^th), the sequence encoded 923 amino acid. Bioinformatics indicated that the GAGP protein’s molecular weight was about 106.9βkD, IEP was 8.42, located in mitochondria. The study also showed expression degree of GAGP was distinct in different cultivars, while it responded obviously to biological and non-biological stress.

Key words: tea plant, GAGP, gene clone, expression

CLC Number:

S571.1
Q52

LI Yuanhua, LU Jianliang, FAN Fangyuan, SHI Yutao. Gene Cloning and Expression Analysis of GAGP in Tea Plant[J]. Journal of Tea Science, 2015, 35(1): 64-72.

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Gene Cloning and Expression Analysis of GAGP in Tea Plant

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