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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (2): 173-181.

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Research on Acute and Subacute Toxicity Evaluation of Liupao Tea

WU Wenliang1,2,3,6, LIN Yong1,2,3,*, LIU Zhonghua1,2,3, HUANG Jian'an1,2,3, LONG Zhirong4,5, TENG Cuiqing4,5, MA Shicheng4,5, QIU Ruijin4,5, CAO Zhonghuan4,5   

  1. 1. Key Lab of Tea Science of Education Ministry, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China;
    4. Wuzhou Academy of Liupao Tea, Wuzhou 543003, China;
    5. Wuzhou Institute of Agricultural Sciences, Wuzhou 543003, China;
    6. Tea of Institute, Hunan Academy of Agricultural Sciences , Changsha 410125, China
  • Received:2017-01-11 Revised:2017-02-26 Online:2017-04-15 Published:2019-08-22

Abstract: Liupao tea has a long history and is characterized as famous agricultural products of Guangxi, but the study of its safety is not enough. In order to verify the safety, the main physicochemical components of Liupao tea from Wuzhou were measured by conventional test method of national standard, then, the acute and subacute toxicities of Liupao tea were systemically studied in accordance with the procedures for toxicological assessment on food safety. The experimental results showed that, the physicochemical indexes of Liupao tea were repeatable, which were followed with Guangxi local standards in the acute toxicity test. Using bliss method, the calculated median lethal dose (LD50) of Liupao tea was 9.38 g·kg-1 with 95% confidence interval (8.43~10.44 g·kg-1), which was higher than acute toxicity criteria (5 g·kg-1). In the subacute study, compared with the control group, no death or clinical signs, or abnormally hematological, biochemical and histopathological changes were found in either groups of rats receiving daily oral gavage of Liupao tea. The three dose treatments were 0.47, 0.94 and 1.88 g·kg-1·d-1 for 30 days (P>0.05). Thus, Liupao tea products belong to the actual non-toxic grade and fulfill the requirements of food safety according to the food toxicology standards.

Key words: Liupao tea, acute toxicity, subacute toxicity, median lethal dosage

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